The Effect of Proflavin, Ethidium Bromide and an Elevated Temperature on the Appearance of Nisin-negative Clones in Nisin-producing Strains of Streptococcus lactis
| Autor: | Marianna Rajchert-Trzpil, Władysław T. Dobrzański, Witold Kozak |
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| Rok vydání: | 1974 |
| Předmět: |
Hot Temperature
Extrachromosomal Inheritance Rifamycins Diamines medicine.disease_cause Microbiology chemistry.chemical_compound Plasmid Ethidium medicine Gene Nisin health care economics and organizations Nitrosoguanidines Mutation biology Strain (chemistry) Lactococcus lactis Drug Resistance Microbial biology.organism_classification Clone Cells chemistry Acridines Ethidium bromide |
| Zdroj: | Journal of General Microbiology. 83:295-302 |
| ISSN: | 0022-1287 |
| DOI: | 10.1099/00221287-83-2-295 |
| Popis: | SUMMARY: In some nisin-producing strains of Streptococcus lactis, clones which were permanently incapable of producing nisin (Nis-) appeared spontaneously with a frequency of 0.03 to 0.82%. Treatment with proflavin (Pro), ethidium bromide (EB) or at a temperature of 40 °C increased the number of such clones. In one strain 78% of cells became Nis- after treatment with Pro. Treatment with nitrosoguanidine did not reverse the effect of Pro or EB. There was no evidence that Pro or EB selected spontaneously-occurring Nis- clones. These observations suggest that the gene(s) responsible for nisin production in some S. lactis strains may be located on a plasmid, but further investigation is required to confirm this. |
| Databáze: | OpenAIRE |
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