Spectroscopic and Molecular Methods to Differentiate Gender in Immature Date Palm (Phoenix dactylifera L.)
Autor: | Ahmed Al-Rawahi, Muhammad Numan, Fazal Mabood, Ahmed Al-Harrasi, Noor Mazin Abdulkareem, Abdul Latif Khan |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Sucrose Phoenix dactylifera Reflectance spectroscopy RT-PCR Plant Science Biology 01 natural sciences transcript accumulation 03 medical and health sciences chemistry.chemical_compound Phylogenetics Botany sex Ecology Evolution Behavior and Systematics PLS regression 030304 developmental biology 0303 health sciences Sexual differentiation Ecology NIR and FTIR spectroscopy chemometrics NMR chemistry QK1-989 Ploidy Palm Fruit tree 010606 plant biology & botany |
Zdroj: | Plants Volume 10 Issue 3 Plants, Vol 10, Iss 536, p 536 (2021) |
ISSN: | 2223-7747 |
DOI: | 10.3390/plants10030536 |
Popis: | Phoenix dactylifera (date palm) is a well-known nutritious and economically important fruit tree found in arid regions of the Middle East and North Africa. Being diploid, it has extremely high divergence in gender, where sex differentiation in immature date palms (Phoenix dactylifera L.) has remained an enigma in recent years. Herein, new robust infrared (near-infrared reflectance spectroscopy (NIRS) and Fourier transform infrared attenuated total reflectance (FTIR/ATR)) and nuclear magnetic resonance (NMR) spectroscopy methods coupled with extensive chemometric analysis were used to identify the sex differentiation in immature date palm leaves. NIRS/FTIR reflectance and 1H-NMR profiling suggested that the signals of monosaccharides (glucose and fructose) and/or disaccharides (maltose and sucrose) play key roles in sex differentiation. The three kinds of spectroscopic data were clearly differentiated among known and unknown male and female leaves via principal component and partial least square discriminant analyses. Furthermore, sex-specific genes and molecular markers obtained from the lower halves of LG12 chromosomes showed enhanced transcript accumulation of mPdIRDP52, mPdIRDP50, and PDK101 in females compared with in males. The phylogeny showed that the mPdIRD033, mPdIRD031, and mPdCIR032 markers formed distinctive clades with more than 70% similarity in gender differentiation. The three robust analyses provide an alternative tool to differentiate sex in date palm trees, which offers a solution to the long-standing challenge of dioecism and could enhance in situ tree propagation programs. |
Databáze: | OpenAIRE |
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