BCG lymphadenopathy detected in a BCG-vaccinated infant
| Autor: | Miriam T. P. Lopes, C.J. Augusto, M. S. Zanini, M V N P Queiroz, Carlos E. Salas, A. S. Barouni |
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| Rok vydání: | 2004 |
| Předmět: |
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Bacterial Male Tuberculosis Physiology Antibiotic resistance Immunology Population Biophysics Lymphadenopathy Biology Biochemistry Polymerase Chain Reaction Amidohydrolases Lymphadenitis medicine Humans General Pharmacology Toxicology and Pharmaceutics education lcsh:QH301-705.5 Ethambutol lcsh:R5-920 Mycobacterium bovis education.field_of_study General Neuroscience Isoniazid Vaccination Cell Biology General Medicine Pyrazinamide biology.organism_classification medicine.disease Virology Polymerase chain reaction Bacille Calmette-Guérin (BCG) lcsh:Biology (General) Child Preschool PncA BCG Vaccine Lymph Nodes lcsh:Medicine (General) Rifampicin Polymorphism Restriction Fragment Length medicine.drug |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Volume: 37, Issue: 5, Pages: 697-700, Published: MAY 2004 Brazilian Journal of Medical and Biological Research, Vol 37, Iss 5, Pp 697-700 (2004) |
| ISSN: | 0100-879X |
| Popis: | Large-scale vaccination with BCG, the live attenuated strain of Mycobacterium bovis, is being adopted around the world, although sporadic complications have occurred after the procedure. Lymphadenopathy is not uncommon especially in babies under one year (0.73% of vaccinated infants), but the swelling subsides within 2 months in most cases, with no medical or surgical treatment. Brazil adopted BCG vaccination program earlier in the seventies and by 1995 more than 96% of the infant population received this immunization. We report here the occurrence of lymphadenopathy in a two-year-old child vaccinated with the Brazilian BCG strain. The diagnosis was made using a lymph node biopsy and intestinal aspirates that yielded a positive mycobacterial culture. The isolate was resistant to isoniazid, rifampicin, pyrazinamide and thiophen-2-carbonic acid hydrazide, sensitive to streptomycin, ethambutol, and p-nitrobenzoic acid, and reacted positively to cyclo-serine and negatively to niacin. The pncA gene involved in bacterial activation of pyrazinamide contains in M. bovis a point mutation that renders pyrazinamidase unable to catalyze drug activation. Therefore, this polymorphism is a good option for developing methods to differentiate M. bovis and M. tuberculosis. Taking advantage of this difference we further analyzed the isolates by single-stranded conformation polymorphism electrophoresis of DNA following PCR of the pncA gene. The isolate identity was confirmed by RFLP electrophoretic analysis of the amplified fragment following Eco065I digestion, which selectively cleaves M. tuberculosis DNA. From this result it is proposed that RFLP of pncA gene represents an alternative for differential diagnosis of M. bovis. |
| Databáze: | OpenAIRE |
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