The effect of two CpG oligodeoxynucleotides with different sequences on haemocytic immune responses of giant freshwater prawn, Macrobrachium rosenbergii
Autor: | Chu-Wen Yang, Po-Tzung Chang, Hung-Hung Sung, Yi-Hsiu Lin |
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Rok vydání: | 2009 |
Předmět: |
Hemocytes
Pyrrolidines CpG Oligodeoxynucleotide Aquatic Science Biology Antioxidants Immune system Adjuvants Immunologic Thiocarbamates Animals Environmental Chemistry RNA Messenger Gene Messenger RNA Macrobrachium rosenbergii hemic and immune systems General Medicine Anatomy Prophenoloxidase respiratory system biology.organism_classification Molecular biology Hedgehog signaling pathway Gene Expression Regulation Oligodeoxyribonucleotides Prawn CpG Islands Palaemonidae |
Zdroj: | Fish & Shellfish Immunology. 26:256-263 |
ISSN: | 1050-4648 |
Popis: | A previous study has demonstrated that the intrahaemocytic phenoloxidase (PO) activity of the prawn Macrobrachium rosenbergii was enhanced by CpG oligodeoxynucleotide (ODN) 2006, but not by os-ODN13. The study described herein determined the binding characteristics of the two ODNs to haemocytes. Treating haemocytes with FAM-ODN or FAM-ODN plus different concentrations of the same ODN revealed that both ODNs specifically bound to haemocytes. Results from haemocytes treated with FAM-ODN2006 plus ROX-os-ODN13 in a competitive assay indicated that about 91% of haemocytes were simultaneously bound by the two ODNs and that the remaining haemocytes were only bound by ODN2006; moreover, ODN2006 binding to haemocytes was stronger than that of os-ODN13. To clarify the interactive effect of the two ODNs on haemocytic function, mRNA levels of haemocytic genes from single or double ODN-injected prawns were determined by semi-quantitative RT-PCR. The expression of either the prophenoloxidase ( proPO ) or peroxinectin ( pon ) genes was elevated by ODN2006 but reduced by os-ODN13; furthermore, ODN2006-increased proPO expression was abated following treatment with os-ODN13. In comparison with ODN-injected prawns alone, the NF-κB inhibitor PDTC reduced the proPO mRNA levels induced by ODN2006, but it elevated proPO expression inhibited by os-ODN13. These results support the notion that os-ODN13 may be able to neutralise or negate the enhancing effect of ODN2006 on proPO expression via the NF-κB signalling pathway as well as an unknown signalling pathway. |
Databáze: | OpenAIRE |
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