Isolation of a Human YAC Contig Encompassing a Cluster of UGT2 Genes and Its Regional Localization to Chromosome 4q13
| Autor: | Douglas J. Clarke, Chee Gee See, Maureen Boxer, Gemma Monaghan, Brian Burchell, Sue Povey |
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| Rok vydání: | 1994 |
| Předmět: |
Genetics
Yeast artificial chromosome Base Sequence Contig Molecular Sequence Data Chromosome Mapping Chromosome Hybrid Cells Biology Polymerase Chain Reaction Chromosome 4 Gene mapping Multigene Family Gene cluster Animals Humans Chromosomes Human Pair 4 Glucuronosyltransferase Chromosome 21 Chromosomes Artificial Yeast Chromosome 22 In Situ Hybridization Fluorescence DNA Primers |
| Zdroj: | Genomics. 23:496-499 |
| ISSN: | 0888-7543 |
| Popis: | Previously the authors mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here they report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes were isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13. 22 refs., 2 figs., 1 tab. |
| Databáze: | OpenAIRE |
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