Capillary electrophoresis-based assay of phosphofructokinase-1
| Autor: | Andrew Malina, Simon H. Chang, S. Douglass Gilman, Sherrisse K. Bryant, Grover L. Waldrop |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Phosphofructokinase-1
Biophysics Biochemistry Article chemistry.chemical_compound Adenosine Triphosphate Capillary electrophoresis Aurintricarboxylic acid Animals Phosphofructokinase 1 Enzyme Inhibitors Molecular Biology Enzyme Assays chemistry.chemical_classification Chromatography biology Chemistry Electrophoresis Capillary Cell Biology Enzyme assay Adenosine Diphosphate Electrophoresis Adenosine diphosphate Enzyme biology.protein Rabbits Adenosine triphosphate |
| Zdroj: | Analytical Biochemistry. 447:1-5 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2013.10.028 |
| Popis: | An assay was developed for phosphofructokinase-1 (PFK-1) using capillary electrophoresis (CE). In the glycolytic pathway, this enzyme catalyzes the rate-limiting step from fructose-6-phosphate and magnesium-bound adenosine triphosphate (Mg-ATP) to fructose-1,6-bisphosphate and magnesium-bound adenosine diphosphate (Mg-ADP). This enzyme has recently become a research target because of the importance of glycolysis in cancer and obesity. The CE assay for PFK-1 is based on the separation and detection by ultraviolet (UV) absorbance at 260 nm of Mg-ATP and Mg-ADP. The separation was enhanced by the addition of Mg²⁺ to the separation buffer. Inhibition studies of PFK-1 by aurintricarboxylic acid and palmitoyl coenzyme A were also performed. An IC₅₀ value was determined for aurintricarboxylic acid, and this value matched values in the literature obtained using coupled spectrophotometric assays. This assay for PFK-1 directly monitors the enzyme-catalyzed reaction, and the CE separation reduces the potential of spectral interference by inhibitors. |
| Databáze: | OpenAIRE |
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