Epithelial-mesenchymal interactions induce enamel matrix proteins and proteases in the epithelial cells of the rests of Malassez in vitro
| Autor: | Masahiko Kikuchi, Rui Wang, Ken Takahashi, Hiroatsu Watanabe, Mitsuru Shimonishi |
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| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Periodontal Ligament Cellular differentiation Morphogenesis Gene Expression In situ hybridization Matrix (biology) Dental Enamel Proteins medicine Periodontal fiber Humans General Dentistry Cells Cultured In Situ Hybridization Amelogenin Chemistry Reverse Transcriptase Polymerase Chain Reaction Regeneration (biology) Mesenchymal stem cell Cell Differentiation Epithelial Cells Fibroblasts Immunohistochemistry Cell biology Matrix Metalloproteinase 20 Kallikreins Peptide Hydrolases |
| Zdroj: | European journal of oral sciences. 120(6) |
| ISSN: | 1600-0722 |
| Popis: | Epithelial-mesenchymal interactions influence morphogenesis and cell differentiation in periodontal tissue regeneration. The current study examined the expression of amelogenin, ameloblastin, matrix metallopeptidase-20 (MMP-20), and kallikrein-4 (KLK-4) and their effects on the interactions between the epithelial cells of Malassez and periodontal ligament fibroblasts. Explants of human periodontal ligament tissues produced outgrowths containing both the epithelial cells of Malassez and periodontal ligament fibroblasts after incubation in a modified serum-free medium. Both the epithelial cells and fibroblasts were co-cultured in the same dish. The distribution and expression of all four factors were evaluated using immunohistochemistry, in-situ hybridization and RT-PCR analysis. The epithelial cells of Malassez were cultured separately and were used as the control. Immunohistochemical analysis revealed weak expression of amelogenin, ameloblastin, MMP-20 and KLK-4 in epithelial cells of Malassez co-cultured with periodontal ligament fibroblasts. in-situ hybridization and RT-PCR confirmed significant mRNA expression of these factors in co-cultured cells compared with control cells. MMP20 mRNA was not expressed in control cells. These results suggest that the epithelial-mesenchymal interactions promote differentiation of human epithelial cells of Malassez and that the induction of enamel matrix proteases facilitates the degradation of enamel matrix proteins. |
| Databáze: | OpenAIRE |
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