Disposable silicon-based all-in-one micro-qPCR for apid on-site detection of pathogens
| Autor: | Michael Kasimatis, Ugur Tanriverdi, Matti Kaisti, Tarek Asfour, Max Grell, Alexander Silva Pinto Collins, Firat Güder, Estefania Nunez-Bajo, Yasin Cotur, Karen Stevenson, Guglielmo Senesi |
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| Přispěvatelé: | Wellcome Trust |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
DEVICES
General Physics and Astronomy 02 engineering and technology medicine.disease_cause 01 natural sciences law.invention law REAL-TIME PCR TEMPERATURE Polymerase chain reaction Sensors and probes Coronavirus Oligonucleotide Array Sequence Analysis Multidisciplinary 021001 nanoscience & nanotechnology Mycobacterium avium subspecies paratuberculosis Publisher Correction Electrical and electronic engineering Sensors and biosensors Multidisciplinary Sciences DNA AMPLIFICATION Mycobacterium avium subsp. paratuberculosis Real-time polymerase chain reaction Science & Technology - Other Topics RNA Viral 0210 nano-technology Nucleic Acid Amplification Techniques DNA Bacterial Silicon POLYMERASE-CHAIN-REACTION Science Loop-mediated isothermal amplification Biology Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology Article ELECTROCHEMISTRY medicine Animals Humans ISOTHERMAL AMPLIFICATION Science & Technology SARS-CoV-2 010401 analytical chemistry COVID-19 General Chemistry Nucleic acid amplification technique biology.organism_classification Virology 0104 chemical sciences genomic DNA Nucleic acid |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020) |
| ISSN: | 2041-1723 |
| Popis: | Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences of nucleic acids. TriSilix can be produced at wafer-scale in a standard laboratory (37 chips of 10 × 10 × 0.65 mm in size can be produced in 7 h, costing ~0.35 USD per device). We are able to quantitatively detect a 563 bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20 fg, equivalent to a single bacterium, at the 35th cycle. Using TriSilix, we also detect the cDNA from SARS-CoV-2 (1 pg) with high specificity against SARS-CoV (2003). Designing efficient, rapid and low-cost diagnostic technologies targeting nucleic acids remains a challenge. Here the authors present a disposable silicon-based integrated Point-of-Need transducer produced in a standard wet lab and able to chemically-amplify and detect pathogen-specific sequences of nucleic acids quantitatively in real-time. |
| Databáze: | OpenAIRE |
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