Modified Mutagen Metabolism inSchistosoma hematobium–Infested Organisms
| Autor: | Harvey D. Blankespoor, Marilyn Aardema, James M. Gentile, Debra Clark, Sherri Brown |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
Aroclors endocrine system Aflatoxin Aflatoxin B1 Liver Diseases Parasitic 3 3'-Dichlorobenzidine Mutagen Urine In Vitro Techniques Biology medicine.disease_cause Microbiology Ames test chemistry.chemical_compound Aflatoxins Cricetinae parasitic diseases medicine Animals Schistosomiasis Environmental Chemistry Biotransformation Carcinogen Glucuronidase General Environmental Science Mesocricetus Mutagenicity Tests Public Health Environmental and Occupational Health food and beverages 2-Acetylaminofluorene Chlorodiphenyl (54% Chlorine) biology.organism_classification Rats Disease Models Animal Liver chemistry Carcinogens Schistosoma haematobium |
| Zdroj: | Archives of Environmental Health: An International Journal. 40:5-12 |
| ISSN: | 0003-9896 |
| DOI: | 10.1080/00039896.1985.10545881 |
| Popis: | The relationship between parasite infestation and chemical mutagen metabolism was investigated in this study. Schistosoma hematobium, long associated with increased incidence of bladder cancer in humans, was chosen as a model parasite. Urine samples, serum samples, and liver tissue extracts (S-9) from infested and control hamsters were used with the Ames Salmonella/microsome test to follow 3,3'-dichlorobenzidine (DCB), aflatoxin B1 (AFB1), and 2-acetylaminofluorene (AAF) mutagenicity. Liver S-9 preparations from infested and control hamsters showed little difference in activation potential for DCB and AFB1. Aroclor 1254-induced rat liver S-9, however, was remarkably efficient at reducing the mutagenicity of DCB. This process was reversible by beta-glucuronidase (BG). Studies on infested and control hamsters indicated increased BG activity in serum and urine. Urine concentrates (UC) from infested and control animals were not mutagenic by themselves, but did enhance the mutagenicity of AAF and DCB in the presence of S-9 and BG. Urine concentrates from infested animals showed greater enhancement of DCB mutagenicity than did UC from control animals. These data suggest that increased BG and unknown urinary factors in infested hamsters play a role in altering chemical mutagen activity. |
| Databáze: | OpenAIRE |
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