ESCRT machinery mediates selective microautophagy of endoplasmic reticulum in yeast
| Autor: | Julia Schessner, Michael Knop, Takuma Tsuji, Dimitrios Papagiannidis, Toyoshi Fujimoto, Katharina Schaeff, Peter W. Bircham, Jasmin A. Schäfer, Oliver Pajonk, Giulia Ruffini, Sebastian Schuck, Charlotta Funaya |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
ESCRT machinery
yeast Endoplasmic Reticulum SACCHAROMYCES-CEREVISIAE 0302 clinical medicine Homeostasis Membrane & Intracellular Transport 0303 health sciences biology MEMBRANE-PROTEINS General Neuroscience PROLIFERATION Nuclear Proteins Articles Endoplasmic Reticulum Stress Cell biology endoplasmic reticulum Phosphatase complex AUTOPHAGY TURNOVER Autophagy & Cell Death Life Sciences & Biomedicine Biochemistry & Molecular Biology microautophagy Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae BIOGENESIS General Biochemistry Genetics and Molecular Biology Article ER-PHAGY 03 medical and health sciences TOMOGRAPHY Organelle Microautophagy Molecular Biology 030304 developmental biology Science & Technology General Immunology and Microbiology RECEPTOR Endosomal Sorting Complexes Required for Transport Endoplasmic reticulum Autophagy Membrane Proteins Cell Biology Intracellular Membranes biology.organism_classification Membrane protein 030217 neurology & neurosurgery Biogenesis SYSTEM |
| Zdroj: | EMBO Journal The EMBO Journal |
| Popis: | ER‐phagy, the selective autophagy of endoplasmic reticulum (ER), safeguards organelle homeostasis by eliminating misfolded proteins and regulating ER size. ER‐phagy can occur by macroautophagic and microautophagic mechanisms. While dedicated machinery for macro‐ER‐phagy has been discovered, the molecules and mechanisms mediating micro‐ER‐phagy remain unknown. Here, we first show that micro‐ER‐phagy in yeast involves the conversion of stacked cisternal ER into multilamellar ER whorls during microautophagic uptake into lysosomes. Second, we identify the conserved Nem1‐Spo7 phosphatase complex and the ESCRT machinery as key components for micro‐ER‐phagy. Third, we demonstrate that macro‐ and micro‐ER‐phagy are parallel pathways with distinct molecular requirements. Finally, we provide evidence that the ESCRT machinery directly functions in scission of the lysosomal membrane to complete the microautophagic uptake of ER. These findings establish a framework for a mechanistic understanding of micro‐ER‐phagy and, thus, a comprehensive appreciation of the role of autophagy in ER homeostasis. Micro‐ER‐phagy requires the Nem1‐Spo7 phosphatase complex and ESCRT proteins for membrane scission during uptake of multilamellar ER whorls into the lysosome. |
| Databáze: | OpenAIRE |
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