Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particles
| Autor: | Jong Hoon Park, Yeon-Gil Choi, Hye-Won Hong, Kyungeun Cha, Gyuha Ryu, Heejoon Myung, Hee-Kwon Chae, Min Joo Lee |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Silver Cell Gene Expression Metal Nanoparticles Bioengineering Inflammation Apoptosis Biology Applied Microbiology and Biotechnology Models Biological Silver nanoparticle chemistry.chemical_compound Mice Cell Line Tumor Gene expression medicine Animals Humans Oligonucleotide Array Sequence Analysis Mice Inbred BALB C Reverse Transcriptase Polymerase Chain Reaction General Medicine Glutathione Molecular biology In vitro Mitochondria medicine.anatomical_structure chemistry Liver Cell culture medicine.symptom Biotechnology |
| Zdroj: | Biotechnology letters. 30(11) |
| ISSN: | 1573-6776 |
| Popis: | Mice were fed either 13 nm silver nanoparticles or 2-3.5 mum silver microparticles. The livers were then obtained after 3 days and subjected to a histopathological analysis. The nanoparticle-fed and microparticle-fed livers both exhibited lymphocyte infiltration in the histopathological analysis, suggesting the induction of inflammation. In vitro, a human hepatoma cell line (Huh-7) was treated with the same silver nanoparticles and microparticles. The mitochondrial activity and glutathione production were hardly affected. However, the DNA contents decreased 15% in the nanoparticle-treated cells and 10% in the microparticle-treated cell, suggesting a more potent induction of apoptosis by the nanoparticles. From a microarray analysis of the RNA from the livers of the nano- and micro-particle-fed mice, the expression of genes related to apoptosis and inflammation was found to be altered. These gene expression changes in the nanoparticle-treated livers lead to phenotypical changes, reflecting increased apoptosis and inflammation. The changes in the gene expression were confirmed by using a semi-quantitative RT-PCR. |
| Databáze: | OpenAIRE |
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