| Autor: |
Eman M. Abd Elghani, Farghaly A. Omar, Marwa M. Abdel-Aziz Emam, Abdulaziz M. Al-Mahallawi, Soad H. Tadros, Fathy M. Soliman, Abeer M. ElSayed |
| Rok vydání: |
2022 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.20427692.v1 |
| Popis: |
Fruits of Citrus sinensis L. Osbeck var. Valencia contain hesperidin as a major flavanone glycoside. Hesperidin (H) was isolated from the peels of Valencia orange and formulated as hexosomal nanodispersions (F1) adopting the hot emulsification method. The antimycobacterial activity(anti-TB) was evaluated through a microplate Alamar blue (MABA) assay where F1 showed significant activity with MIC = 0.19 µM. To unravel the potential mechanism of the anti-TB, a molecular docking study of H using the Mycobacterial Dihydrofolate reductase (Mtb. DHFR) enzyme was performed. Hesperidin exhibited significant interactions with Mtb. DHFR active site. Sulforhodamine B assay was applied to evaluate cytotoxic activity against the lung cancer cell line (A-549). F1 showed a cytotoxic effect at IC50= 33 µM. It also has potent antiviral activity against Human Coronavirus 229E with IC50= 258.8 μM utilising crystal violet assay. Peels of Valencia orange could be a source of bioactive metabolites to control significant diseases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
