Paracrine effect of human adipose-derived stem cells on lymphatic endothelial cells
| Autor: | Ludovica Barone, Cristiana Marcozzi, Marina Borgese, Roberto Valli, Eleonora Solari, Rosalba Gornati, A. Frattini, Federica Rossi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Embryology
government.form_of_government Vascular Endothelial Growth Factor C Biomedical Engineering Biology 03 medical and health sciences Paracrine signalling 0302 clinical medicine Adipocytes Animals Humans Lymphangiogenesis 030304 developmental biology Lymphatic Vessels 0303 health sciences Stem Cells lymphangiogenesis conditioned medium gene expression ELISA immunohistochemistry Endothelial Cells Vascular Endothelial Growth Factor Receptor-3 Rats Endothelial stem cell Lymphatic Endothelium Vascular endothelial growth factor A Lymphatic system Vascular endothelial growth factor C 030220 oncology & carcinogenesis government Cancer research Stem cell |
| Zdroj: | Regenerative medicine. 15(9) |
| ISSN: | 1746-076X |
| Popis: | Aim: The proposal of this study was to evaluate, in vitro, the potential paracrine effect of human adipose-derived stem cells (hASCs) to promote lymphangiogenesis in lymphatic endothelial cells isolated from rat diaphragmatic lymphatic vessels. Materials & methods: ELISA on VEGFA, VEGFC and IL6 in hASC-conditioned medium; LYVE1 immunostaining; and gene expression of PROX1, VEGFR3, VEGFC, VEGFA and IL6 were the methods used. Results: In 2D culture, hASC-conditioned medium was able to promote lymphatic endothelial cell survival, maintenance of endothelial cobblestone morphology and induction to form a vessel-like structure. Conclusion: The authors' results represent in vitro evidence of the paracrine effect of hASCs on lymphatic endothelial cells, suggesting the possible role of hASC-conditioned medium in developing new therapeutic approaches for lymphatic system-related dysfunction such as secondary lymphedema. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|