Effect of Cerenkov Radiation-Induced Photodynamic Therapy with 18F-FDG in an Intraperitoneal Xenograft Mouse Model of Ovarian Cancer

Autor: Jia Je Li, Ren Shyan Liu, Chien Chih Ke, Yi An Chen, Syue Liang Lin, Sain Jhih Chiu, Chi Wei Chang, Bang Hung Yang, Ming Cheng Chang, Fong Shya Jeng, Cheng Hsiu Lu
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
photosensitizer
medicine.medical_treatment
Photodynamic therapy
chemistry.chemical_compound
0302 clinical medicine
Photosensitizer
Biology (General)
Spectroscopy
Ovarian Neoplasms
Mice
Inbred BALB C
Radiation
medicine.diagnostic_test
Singlet oxygen
General Medicine
Verteporfin
Computer Science Applications
Chemistry
ovarian cancer
photodynamic therapy
Positron emission tomography
030220 oncology & carcinogenesis
Female
Injections
Intraperitoneal
medicine.drug
QH301-705.5
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
In vivo
Fluorodeoxyglucose F18
Cerenkov radiation
Cell Line
Tumor
medicine
Animals
Viability assay
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
Organic Chemistry
18F-FDG
medicine.disease
Xenograft Model Antitumor Assays
030104 developmental biology
chemistry
Photochemotherapy
Cancer research
Ovarian cancer
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 9
International Journal of Molecular Sciences, Vol 22, Iss 4934, p 4934 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22094934
Popis: Ovarian cancer (OC) metastases frequently occur through peritoneal dissemination, and they contribute to difficulties in treatment. While photodynamic therapy (PDT) has the potential to treat OC, its use is often limited by tissue penetration depth and tumor selectivity. Herein, we combined Cerenkov radiation (CR) emitted by 18F-FDG accumulated in tumors as an internal light source and several photosensitizer (PS) candidates with matched absorption bands, including Verteporfin (VP), Chlorin e6 (Ce6) and 5′-Aminolevulinic acid (5′-ALA), to evaluate the anti-tumor efficacy. The in vitro effect of CR-induced PDT (CR-PDT) was evaluated using a cell viability assay, and the efficiency of PS was assessed by measuring the singlet oxygen production. An intraperitoneal ES2 OC mouse model was used for in vivo evaluation of CR-PDT. Positron emission tomography (PET) imaging and bioluminescence-based imaging were performed to monitor the biologic uptake of 18F-FDG and the therapeutic effect. The in vitro studies demonstrated Ce6 and VP to be more effective PSs for CR-PDT. Moreover, VP was more efficient in the generation of singlet oxygen and continued for a long time when exposed to fluoro-18 (18F). Combining CR emitted by 18F-FDG and VP treatment not only significantly suppressed tumor growth, but also prolonged median survival times compared to either monotherapy.
Databáze: OpenAIRE
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