Developmental Aberrations of Liver Gene Expression in Bovine Fetuses Derived from Somatic Cell Nuclear Transplantation
| Autor: | Seiya Takahashi, Chandana B Herath, Kei Imai, Akira Hirasawa, Kazuyoshi Hashizume, Toru Takahashi, Satoshi Shiojima, Yoshiaki Izaike, Gozoh Tsujimoto, Koichi Ushizawa, Tadashi Kadowaki, Hiroko Ishiwata, Susumu Katsuma |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cloning
Fetus DNA Complementary Microarray Somatic cell Cloning Organism Gene Expression Profiling Biology Molecular biology Gene expression profiling Liver Pregnancy Complementary DNA embryonic structures Gene expression Animals Somatic cell nuclear transfer Cattle Female Oligonucleotide Array Sequence Analysis Developmental Biology Biotechnology |
| Zdroj: | Cloning and Stem Cells. 8:79-95 |
| ISSN: | 1557-7457 1536-2302 |
| DOI: | 10.1089/clo.2006.8.79 |
| Popis: | Cloning by somatic cell nuclear transfer (NT) has been accomplished. However, the process itself is inefficient since most clones die before birth and survivors often display various anomalies. In an effort to determine global expression profiles of developmentally regulated liver genes in NT bovine fetuses, we employed a custom-made bovine liver complementary DNA (cDNA) microarray. The NT fetuses in early pregnancy were derived from cumulus cells as the nuclear donor cells. Normal fetuses were derived from in vitro fertilization (IVF) and artificial insemination (AI). Gene expression levels in NT, IVF, and AI fetal livers were obtained by comparing individual fetal liver samples with that of adult liver of nonpregnant cycling cows. Statistical analyses of the expression data showed widespread dysregulation of developmentally important genes in the three NT fetuses examined. It was found that the number of dysregulated genes was within a range of 3.5-7.7% of the tested genes in the NT fetal livers. The analyses revealed that one NT fetus was markedly different in liver gene expression profile from the other two NT fetal livers in which the expression profiles were highly correlated. Thus, our findings demonstrate that widespread dysregulation of liver genes occurs in the developing liver of NT bovine fetuses. It is possible that inappropriate genomic reprogramming after NT is a key factor associated with abnormal gene expressions in the livers of NT fetuses, whereas distinct expression patterns between the fellow cloned fetuses likely have resulted from variable epigenetic status of the donor nuclei. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|