Involvement of the leaf-specific multidrug and toxic compound extrusion (MATE) transporter Nt-JAT2 in vacuolar sequestration of nicotine in Nicotiana tabacum

Autor: Yoshinori Moriyama, Kojiro Takanashi, Masahiko Morita, Minaho Hayashida, Alain Goossens, Shingo Ito, Kazufumi Yazaki, Nobukazu Shitan, Akifumi Sugiyama, Shota Minami, Masataka Moriyasu, Hiroshi Omote
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Pyridines
Nicotiana tabacum
lcsh:Medicine
Plant Science
Vacuole
Acetates
Biochemistry
Plant Roots
SECONDARY METABOLISM
chemistry.chemical_compound
CATHARANTHUS-ROSEUS
Plant defense against herbivory
Plant Hormones
PLANT-CELLS
lcsh:Science
Phylogeny
Plant Proteins
COPTIS-JAPONICA
TOBACCO
Multidisciplinary
Methyl jasmonate
Plant Biochemistry
Alkaloid
food and beverages
Anabasine
ARABIDOPSIS
Up-Regulation
BINDING CASSETTE PROTEIN
Plant Physiology
Cellular Structures and Organelles
Cellular Types
Solute Transport
Research Article
Anatabine
Plant Vacuoles
Nicotine
Plant Cell Biology
Cyclopentanes
Saccharomyces cerevisiae
Biology
Alkaloids
Plant Cells
ALKALOID METABOLISM
Tobacco
Oxylipins
RNA
Messenger
Nicotiana
Flavonoids
Gene Expression Profiling
Cell Membrane
fungi
lcsh:R
Biology and Life Sciences
Cell Biology
MEDICAGO-TRUNCATULA
biology.organism_classification
Hormones
Plant Leaves
chemistry
MEMBRANE-TRANSPORT
Vacuoles
lcsh:Q
Zdroj: PLoS ONE, Vol 9, Iss 9, p e108789 (2014)
PLOS ONE
PLoS ONE
ISSN: 1932-6203
Popis: Alkaloids play a key role in higher plant defense against pathogens and herbivores. Following its biosynthesis in root tissues, nicotine, the major alkaloid of Nicotiana species, is translocated via xylem transport toward the accumulation sites, leaf vacuoles. Our transcriptome analysis of methyl jasmonate-treated tobacco BY-2 cells identified several multidrug and toxic compound extrusion (MATE) transporter genes. In this study, we characterized a MATE gene, Nicotiana tabacum jasmonate-inducible alkaloid transporter 2 (Nt-JAT2), which encodes a protein that has 32% amino acid identity with Nt-JAT1. Nt-JAT2 mRNA is expressed at a very low steady state level in whole plants, but is rapidly upregulated by methyl jasmonate treatment in a leaf-specific manner. To characterize the function of Nt-JAT2, yeast cells were used as the host organism in a cellular transport assay. Nt-JAT2 was localized at the plasma membrane in yeast cells. When incubated in nicotine-containing medium, the nicotine content in Nt-JAT2-expressing cells was significantly lower than in control yeast. Nt-JAT2-expressing cells also showed lower content of other alkaloids like anabasine and anatabine, but not of flavonoids, suggesting that Nt-JAT2 transports various alkaloids including nicotine. Fluorescence assays in BY-2 cells showed that Nt-JAT2-GFP was localized to the tonoplast. These findings indicate that Nt-JAT2 is involved in nicotine sequestration in leaf vacuoles following the translocation of nicotine from root tissues.
Databáze: OpenAIRE
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