The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence

Autor: Giuliano Degrassi, Valéria Carpentieri-Pípolo, Vibha Bhardwaj, Ivica Dimkić, Nemanja Kuzmanović
Přispěvatelé: IVICA DIMKIĆ, Department of Biochemistry and Molecular Biology, University of Belgrade – Faculty of Biology, Belgrade, Serbia, VIBHA BHARDWAJ, Ras Al Khaimah Municipality Department, Director Environment Laboratories, Dubai, United Arab Emirate, VALERIA CARPENTIERI PIPOLO, CNPT, NEMANJA KUZMANOVIĆ, Federal Research Centre for Cultivated Plants (JKI), Institute for Plant Protection in Horticulture and Forests, Julius Ku¨ hn-Institut, Braunschweig, Germany, GIULIANO DEGRASSI, Industrial Biotechnology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Buenos Aires, Argentina.
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Polymers
Hydrolases
Glycobiology
Chitin
Genome
Biochemistry
Glycoside hydrolase
N-Glycosyl Hydrolases
Materials
Phylogeny
Data Management
2. Zero hunger
0303 health sciences
Multidisciplinary
biology
Organic Compounds
Hydrolysis
Chitinases
High-Throughput Nucleotide Sequencing
Agriculture
Genomics
Elicitor
Enzymes
Actinobacteria
Phylogenetics
Chemistry
Macromolecules
Physical Sciences
Medicine
Curtobacterium sp
Research Article
Computer and Information Sciences
Phosphorylases
Science
Materials Science
Carbohydrates
Crops
03 medical and health sciences
Bacterial Proteins
Polysaccharides
Transferases
Genetics
Evolutionary Systematics
Gene
030304 developmental biology
Taxonomy
Whole genome sequencing
Evolutionary Biology
Whole Genome Sequencing
030306 microbiology
Organic Chemistry
Chemical Compounds
Biology and Life Sciences
Proteins
biology.organism_classification
Polymer Chemistry
GD1
Plant Leaves
Enzyme
Chitinase
biology.protein
Enzymology
Soybeans
Bacteria
Curtobacterium
Genome
Bacterial
Crop Science
Zdroj: PLoS ONE, Vol 16, Iss 11 (2021)
PLoS ONE, Vol 16, Iss 11, p e0259465 (2021)
PLoS ONE
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
ISSN: 1932-6203
Popis: Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader. Made available in DSpace on 2021-12-06T14:00:59Z (GMT). No. of bitstreams: 1 journal.pone.0259465-PLOSONE-2021.pdf: 2753754 bytes, checksum: c3da2c9b24e14885487e914ee21e59d5 (MD5) Previous issue date: 2021
Databáze: OpenAIRE
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