The Arcanobacterium pyogenes Collagen-Binding Protein, CbpA, Promotes Adhesion to Host Cells
| Autor: | Stephen J. Billington, Malen A. Link, J. Glenn Songer, Paula A. Esmay, B. Helen Jost |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Immunology Mutant Biology Arcanobacterium pyogenes Microbiology Bacterial Adhesion Cell Line Mice Plasmid Bacterial Proteins Animals Humans Amino Acid Sequence Far-western blotting Cloning Molecular Base Sequence Sequence Homology Amino Acid Binding protein Molecular Pathogenesis Antibodies Bacterial Molecular biology Fibronectin Mutagenesis Insertional Infectious Diseases Genes Bacterial Cell culture Actinomycetaceae biology.protein Parasitology Collagen MSCRAMM Carrier Proteins HeLa Cells |
| Zdroj: | Infection and Immunity. 71:4368-4374 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Arcanobacterium pyogenes is an opportunistic pathogen associated with suppurative diseases in economically important food animals such as cattle, pigs, and turkeys. A. pyogenes adheres to host epithelial cells, and adhesion is promoted by the action of neuraminidase, which is expressed by this organism. However, a neuraminidase-deficient mutant of A. pyogenes only had a reduced ability to adhere to host epithelial cells, indicating that other factors are involved in adhesion. Far Western blotting revealed the presence of an approximately 120-kDa A. pyogenes cell wall protein that binds collagen type I. The 3.5-kb gene that encodes the 124.7-kDa CbpA protein was cloned, and sequence analysis indicated that CbpA contains a typical MSCRAMM protein domain structure. Recombinant, six-His-tagged CbpA (HIS-CbpA) was capable of binding collagen types I, II, and IV but not fibronectin. In addition, CbpA was involved in the ability of A. pyogenes to adhere to HeLa and 3T6 cells, as a cbpA knockout strain had 38.2 and 57.0% of wild-type adhesion, respectively. This defect could be complemented by providing cbpA on a multicopy plasmid. Furthermore, HIS-CbpA blocked A. pyogenes adhesion to HeLa or 3T6 cells in a dose-dependent manner. cbpA was only present in 48% of the A. pyogenes strains tested ( n = 75), and introduction of plasmid-encoded cbpA into a naturally cbpA -deficient strain increased the ability of this strain to bind to HeLa and 3T6 cells 2.9- and 5.7-fold, respectively. These data indicate that CbpA, a collagen-binding protein of A. pyogenes , plays a role in the adhesion of this organism to host cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|