| Autor: |
Lisa Tripodi, Elena Pérez Ruiz, Phalguni Tewari Kumar, Tadej Kokalj, Deborah Decrop, Jeroen Lammertyn |
| Přispěvatelé: |
Taly, V, Viovy, JL, Descroix, S |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Methods in Molecular Biology ISBN: 9781493967322 |
| Popis: |
Digital microfluidics has emerged in the last years as a promising liquid handling technology for a variety of applications. Here, we describe in detail how to build up an electrowetting-on-dielectric-based digital microfluidic chip with unique advantages for performing single-molecule detection. We illustrate how superparamagnetic particles can be printed with very high loading efficiency (over 98 %) and single-particle resolution in the microwell array patterned in the Teflon-AF® surface of the grounding plate of the chip. Finally, the potential of the device for its application to single-molecule detection is demonstrated by the ultrasensitive detection of the biotinylated enzyme β-Galactosidase captured on streptavidin-coated particles in the described platform. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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