Development of a Novel One-Step Automated Rapid in situ Hybridization for Anaplastic Lymphoma Kinase Rearrangement Using Non-Contact Alternating-Current Electric-Field Mixing

Autor:	Yoshihiro Minamiya, Shinogu Takashima, Kazuhiro Imai, Satoru Motoyama, Yoichi Akagami, Kaori Terata, Ryuta Nakamura, Tsubasa Matsuo, Akiyuki Wakita, Shin-nosuke Watanabe, Yusuke Sato, Satoshi Fujishima, Hiroshi Nanjo
Rok vydání:	2020
Předmět:	Diagnostic methods In situ hybridization Biology Pathology and Forensic Medicine Fusion gene 03 medical and health sciences 0302 clinical medicine Electricity Carcinoma Non-Small-Cell Lung hemic and lymphatic diseases medicine Humans Anaplastic lymphoma kinase Anaplastic Lymphoma Kinase Lung cancer Molecular Biology In Situ Hybridization Fluorescence Automation Laboratory Gene Rearrangement medicine.diagnostic_test ALK Gene Rearrangement Cell Biology General Medicine medicine.disease 030220 oncology & carcinogenesis Mutation Cancer research Standard protocol 030215 immunology Fluorescence in situ hybridization
Zdroj:	Pathobiology. 87:45-50
ISSN:	1423-0291 1015-2008
Popis:	Echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase (ALK) fusion gene rearrangement is a key driver mutation in non-small cell lung cancer (NSCLC). Although Break-Apart ALK fluorescence in situ hybridization (FISH) is a reliable diagnostic method for detecting ALK gene rearrangement, it is also costly and time-consuming to use as a routine screening test. Our aim was to evaluate the clinical utility of a novel one-step, automated, rapid FISH (Auto-RaFISH) method developed to facilitate hybridization. This method takes advantage of the non-contact mixing effect of an alternating-current electric field. Ten representative specimens from 85 patients diagnosed at multiple centers with primary lung cancer with identified ALK-FISH status were collected. The specimens were all tested using FISH, RaFISH, and Auto-RaFISH. With both RaFISH protocols, the ALK test was completed within 4.5 h, as compared to the 20 h needed for the standard protocol. We found 100% agreement between the standard FISH, RaFISH, and new Auto-RaFISH based on the ALK status, and all methods stained equally well. These findings suggest that Auto-RaFISH could potentially serve as an automated clinical tool for prompt determination of ALK status in NSCLC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e29c2758d9e1dbadec77f48b16c6ca2b https://doi.org/10.1159/000505631 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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