Confined detection volume of fluorescence correlation spectroscopy by bare fiber probes
| Autor: | Franck H. Lei, Jean-François Angiboust, Michel Manfait, Guowei Lu |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
business.industry
Confocal Biophysics Reproducibility of Results Fluorescence correlation spectroscopy General Medicine Equipment Design Microscopy Atomic Force Sensitivity and Specificity law.invention Rhodamine 6G Coupling (electronics) Equipment Failure Analysis chemistry.chemical_compound Optics Spectrometry Fluorescence chemistry Optical microscope law Fiber Optic Technology Near-field scanning optical microscope Fiber business Order of magnitude |
| Zdroj: | European biophysics journal : EBJ. 39(5) |
| ISSN: | 1432-1017 |
| Popis: | A fiber-tip-based near-field fluorescence correlation spectroscopy (FCS) has been developed for confining the detection volume to sub-diffraction-limited dimensions. This near-field FCS is based on near-field illumination by coupling a scanning near-field optical microscope (SNOM) to a conventional confocal FCS. Single-molecule FCS analysis at 100 nM Rhodamine 6G has been achieved by using bare chemically etched, tapered fiber tips. The detection volume under control of the SNOM system has been reduced over one order of magnitude compared to that of the conventional confocal FCS. Related factors influencing the near-field FCS performance are investigated and discussed in detail. In this proof-of-principle study, the preliminary experimental results suggest that the fiber-tip-based near-field FCS might be a good alternative to realize localized analysis at the single-molecule level. |
| Databáze: | OpenAIRE |
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