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This study was conducted to evaluate the effect of a blend of vegetable oils and active ingredients (Ruminatus® - safeeds@safeeds.com.br) on ruminal population density of Prevotella albensis, Prevotella bryantii and Prevotella ruminicola. The blend contained clove (Eugenia caryophyllata), castor (Ricinus communis) and cashew (Anacardium occidentale) oils and microencapsulated active ingredients (eugenol, thymol and vanillin). The experiments were designed using each bacteria exposed to doses of 1.5; 3.0; 4.5 and 6.0 mg/ml of the product and having six replicates. Bacteria were cultivated on Hobson’s M2 medium in Hungate tubes. Cultures cultivated without the addition of any oils were considered as control. Bacteria were cultivated overnight and freshly inoculated into new tubes containing medium. The antimicrobial activity was evaluated using a Spectrophotometer Evolution 201 UV-visible (Thermo Scientific) at 600 nm. Readings were performed at 0, 8, 12 and 24 hours after inoculation. Data were analyzed using the linear mixed models and the NLME Procedure in R software. The use of the blend reduced (P < 0.05) the population density of P. albensis and P. bryantii in comparison to the control treatment. P. ruminicola population only decreased (P < 0.05) when 3.0 mg/ml of the blend was used. Growth impairment of Prevotellaceae prevent excessive degradation of dietary protein, increasing protein digestibility. In addition, there is a reduction on ammonia synthesis, the end product of proteolysis, which is excreted in animal waste. Therefore, the combined action of clove, castor and castor essential oils and microencapsulated active ingredients influences the growth of Gram-negative bacteria and can be used as a rumen modulator. |
