Development and Validation of a Multiplex Non-HLA Antibody Assay for the Screening of Kidney Transplant Recipients
| Autor: | Elena G. Kamburova, Tineke Kardol-Hoefnagel, Bram W. Wisse, Irma Joosten, Wil A. Allebes, Arnold van der Meer, Luuk B. Hilbrands, Marije C. Baas, Eric Spierings, Cornelis E. Hack, Franka E. van Reekum, Arjan D. van Zuilen, Marianne C. Verhaar, Michiel L. Bots, Adriaan C. A. D. Drop, Loes Plaisier, Jan Meeldijk, Niels Bovenschen, Marc A. J. Seelen, Jan Stephan Sanders, Bouke G. Hepkema, Annechien J. A. Lambeck, Laura B. Bungener, Caroline Roozendaal, Marcel G. J. Tilanus, Christina E. Voorter, Lotte Wieten, Elly M. van Duijnhoven, Mariëlle A. C. J. Gelens, Maarten H. L. Christiaans, Frans J. van Ittersum, Shaikh A. Nurmohamed, Neubury M. Lardy, Wendy Swelsen, Karlijn A. M. I. van der Pant, Neelke C. van der Weerd, Ineke J. M. ten Berge, Frederike J. Bemelman, Paul J. M. van der Boog, Johan W. de Fijter, Michiel G. H. Betjes, Sebastiaan Heidt, Dave L. Roelen, Frans H. Claas, Henny G. Otten |
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| Přispěvatelé: | Internal Medicine, Laboratory Medicine, Epidemiology and Data Science, Nephrology, ACS - Atherosclerosis & ischemic syndromes, ACS - Diabetes & metabolism, AII - Inflammatory diseases, RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, MUMC+: DA Transplantatie Immunologie (5), MUMC+: DA TI Staf (9), MUMC+: DA TI Laboratorium (9), MUMC+: MA Nefrologie (9), Interne Geneeskunde, RS: NUTRIM - R3 - Respiratory & Age-related Health, AII - Amsterdam institute for Infection and Immunity, APH - Aging & Later Life, Groningen Institute for Organ Transplantation (GIOT), Groningen Kidney Center (GKC) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Graft Rejection Isoantigens 030230 surgery Kidney Organ transplantation 0302 clinical medicine Isoantibodies Methods Immunology and Allergy Multiplex Kidney transplantation RISK biology CROSS-MATCH TEST Histocompatibility Testing Graft Survival Allografts multiplex assay II TYPE-1 RECEPTOR Antibody protein production Inflammatory diseases Radboud Institute for Molecular Life Sciences [Radboudumc 5] lcsh:Immunologic diseases. Allergy kidney transplant medicine.medical_specialty Immunology Other Research Radboud Institute for Molecular Life Sciences [Radboudumc 0] Human leukocyte antigen HaloTag ANTIGENS 03 medical and health sciences All institutes and research themes of the Radboud University Medical Center MEDIATED REJECTION Antigen TARGETS Journal Article medicine Luminex Humans non-HLA antibody IDENTIFICATION business.industry medicine.disease Kidney Transplantation Transplant Recipients High-Throughput Screening Assays Transplantation 030104 developmental biology biology.protein Kidney Failure Chronic Renal disorders Radboud Institute for Health Sciences [Radboudumc 11] lcsh:RC581-607 business Kidney disease |
| Zdroj: | Frontiers in Immunology Frontiers in Immunology, 9:3002. Frontiers Media S.A. Frontiers in Immunology, 9. Frontiers Media S.A. Frontiers in Immunology, 9:3002, 1-12. Frontiers Media S.A. Kamburova, E G, Kardol-Hoefnagel, T, Wisse, B W, Joosten, I, Allebes, W A, van der Meer, A, Hilbrands, L B, Baas, M C, Spierings, E, Hack, C E, van Reekum, F E, van Zuilen, A D, Verhaar, M C, Bots, M L, Drop, A C A D, Plaisier, L, Meeldijk, J, Bovenschen, N, Seelen, M A J, Sanders, J S, Hepkema, B G, Lambeck, A J A, Bungener, L B, Roozendaal, C, Tilanus, M G J, Voorter, C E, Wieten, L, van Duijnhoven, E M, Gelens, M A C J, Christiaans, M H L, van Ittersum, F J, Nurmohamed, S A, Lardy, N M, Swelsen, W, van der Pant, K A M I, van der Weerd, N C, Ten Berge, I J M, Bemelman, F J, van der Boog, P J M, de Fijter, J W, Betjes, M G H, Heidt, S, Roelen, D L, Claas, F H & Otten, H G 2018, ' Development and Validation of a Multiplex Non-HLA Antibody Assay for the Screening of Kidney Transplant Recipients ', Frontiers in Immunology, vol. 9 . https://doi.org/10.3389/fimmu.2018.03002 Frontiers in immunology, 9. Frontiers Media S.A. Frontiers in Immunology, 9:3002. Frontiers Media SA Frontiers in Immunology, 9 Frontiers in Immunology, Vol 9 (2018) Frontiers in Immunology, 9. Frontiers Media S. A. |
| ISSN: | 1664-3224 |
| Popis: | The best treatment for patients with end-stage renal disease is kidney transplantation. Although graft survival rates have improved in the last decades, patients still may lose their grafts partly due to the detrimental effects of donor-specific antibodies (DSA) against human leukocyte antigens (HLA) and to a lesser extent also by antibodies directed against non-HLA antigens expressed on the donor endothelium. Assays to detect anti-HLA antibodies are already in use for many years and have been proven useful for transplant risk stratification. Currently, there is a need for assays to additionally detect multiple non-HLA antibodies simultaneously in order to study their clinical relevance in solid organ transplantation. This study describes the development, technical details and validation of a high-throughput multiplex assay for the detection of antibodies against 14 non-HLA antigens coupled directly to MagPlex microspheres or indirectly via a HaloTag. The non-HLA antigens have been selected based on a literature search in patients with kidney disease or following transplantation. Due to the flexibility of the assay, this approach can be used to include alternative antigens and can also be used for screening of other organ transplant recipients, such as heart and lung. |
| Databáze: | OpenAIRE |
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