Heteroplasmy due to coexistence of mtCOI haplotypes from different lineages of theThrips tabacicryptic species group
| Autor: | S. J. Gawande, Alana L. Jacobson, A. A. Ingle, Sivalingam Anandhan, Ramasamy Asokan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Arrhenotoky Genetics Mitochondrial DNA Species complex Thysanoptera Haplotype Introgression General Medicine Biology DNA Mitochondrial Heteroplasmy Electron Transport Complex IV 03 medical and health sciences 030104 developmental biology Haplotypes Insect Science Animals Thelytoky Agronomy and Crop Science Phylogeny Hybrid |
| Zdroj: | Bulletin of Entomological Research. 107:534-542 |
| ISSN: | 1475-2670 0007-4853 |
| DOI: | 10.1017/s0007485317000025 |
| Popis: | Heteroplasmy is the existence of multiple mitochondrial DNA haplotypes within the cell. Although the number of reports of heteroplasmy is increasing for arthropods, the occurrence, number of variants, and origins are not well studied. In this research, the occurrence of heteroplasmy was investigated inThrips tabaci, a putative species complex whose lineages can be distinguished by their mitochondrial DNA haplotypes. The results from this study showed that heteroplasmy was due to the occurrence of mitochondrial cytochrome oxydase I (mtCOI) haplotypes from two differentT. tabacilineages. An assay using flow cytometry and quantitative real-time PCR was then used to quantify the per cell copy number of the two mtCOI haplotypes present in individuals exhibiting heteroplasmy from nine geographically distant populations in India. All of theT. tabaciindividuals in this study were found to exhibit heteroplasmy, and in every individual the per cell copy number of mtCOI from lineage 3 comprised 75–98% of the haplotypes detected and was variable among individuals tested. There was no evidence to suggest that the presense of lineage-specific haplotypes was due to nuclear introgression; however, further studies are needed to investigate nuclear introgression and paternal leakage during rare interbreeding between individuals from lineages 2 and 3. |
| Databáze: | OpenAIRE |
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