Interaction of wheat high-mobility-group proteins with four-way-junction DNA and characterization of the structure and expression of HMGA gene
| Autor: | Keng-Hock Pwee, R. Manjunatha Kini, Wensheng Zhang, Qiang Wu |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
DNA
Plant Spermidine Molecular Sequence Data Biophysics Biology Biochemistry Histones Non-histone protein Histone H1 Gene Expression Regulation Plant HMGB Proteins Complementary DNA Magnesium Protein–DNA interaction Amino Acid Sequence HMGA Proteins Molecular Biology Triticum Plant Proteins Base Sequence Dose-Response Relationship Drug Sodium High Mobility Group Proteins HMGA Nuclear Proteins Exons Hmg protein Molecular biology Introns Protein Structure Tertiary DNA-Binding Proteins High-mobility group AT-Hook Motifs Cell Division Protein Binding |
| Zdroj: | Archives of Biochemistry and Biophysics. 409:357-366 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/s0003-9861(02)00630-6 |
| Popis: | Plant high-mobility-group (HMG) chromosomal proteins are the most abundant and ubiquitous nonhistone proteins found in the nuclei of higher eukaryotes. There are only two families of HMG proteins, namely, HMGA and HMGB in plants. The cDNA encoding wheat HMGa protein was isolated and characterized. Wheat HMGA cDNA encodes a protein of 189 amino acid residues. At its N terminus, there is a histone H1-like structure, which is a common feature of plant HMGA proteins, followed by four AT-hook motifs. Polymerase chain reaction results show that the gene contains a single intron of 134 bp. All four AT-hook motifs are encoded by the second exon. Northern blot results show that the expression of HMGA gene is much higher in organs undergoing active cell proliferation. Gel retardation analysis show that wheat HMGa, b, c and histone H1 bind to four-way-junction DNA with high binding affinity, but affinity is dramatically reduced with increasing Mg 2+ and Na + ion concentration. Competition binding studies show that proteins share overlapping binding sites on four-way-junction DNA. HMGd does not bind to four-way-junction DNA. |
| Databáze: | OpenAIRE |
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