Assessment of mismatch repair deficiency, CDX2, beta-catenin and E-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study

Autor: Ancuţa Cutaş, Carmen Stanca Melincovici, Mariana Mărginean, Aranka Ilea, Carmen Mihaela Mihu, Adriana Elena Bulboacă, Elena Mihaela Jianu, Maria Neag, Adina Bianca Boşca, Ioana Maria Moldovan, Sergiu Susman
Rok vydání: 2021
Předmět:
0301 basic medicine
congenital
hereditary
and neonatal diseases and abnormalities
Embryology
Colorectal cancer
Biology
MLH1
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
Neoplastic Syndromes
Hereditary
PMS2
medicine
Humans
CDX2 Transcription Factor
adhesion molecules
neoplasms
Survival rate
beta Catenin
Mismatch Repair Endonuclease PMS2
Original Paper
Brain Neoplasms
nutritional and metabolic diseases
Microsatellite instability
Cell Biology
General Medicine
Cadherins
Prognosis
mismatch repair proteins
medicine.disease
digestive system diseases
MSH6
MutS Homolog 2 Protein
030104 developmental biology
colon cancer
MSH2
CDX2
030220 oncology & carcinogenesis
Colonic Neoplasms
Cancer research
microsatellite instability
DNA mismatch repair
Colorectal Neoplasms
Developmental Biology
Zdroj: Romanian Journal of Morphology and Embryology
ISSN: 2066-8279
1220-0522
DOI: 10.47162/rjme.61.3.10
Popis: Microsatellite instability (MSI) or the deficiency of mismatch repair (MMR) proteins is one of the molecular pathways of colorectal tumorigenesis and may have important clinical implications in predicting the treatment response. We evaluated the relationship between clinicopathological features and MMR proteins [mutL homologue 1 (MLH1), mutS homologue 2 (MSH2), mutS homologue 6 (MSH6), postmeiotic segregation increased 2 (PMS2)], adhesion molecules (E-cadherin, beta-catenin) and caudal-type homeobox 2 (CDX2) in 31 patients with colon adenocarcinoma, using immunohistochemistry. We also aimed to assess the prognostic value of the studied proteins. MLH1 loss was correlated to PMS2 loss (p=0.006) and MSH2 loss (p=0.023); MSH2 loss was significantly associated to MSH6 loss (p=0.011). Tumors with MSH6 loss, together with tumors with PMS2 loss, covered all the patients with MSI status. We found a significant correlation between MSI tumors and mucinous histological type (p=0.03), but no significant associations with other clinicopathological features or with survival rate. There was a significant correlation between E-cadherin expression and differentiation degree (p=0.018) and between beta-catenin expression and lymph node invasion (p=0.046). No significant association between CDX2 loss and any clinical or pathological features was found (p>0.05). No significant differences were identified in overall survival according to E-cadherin, beta-catenin or CDX2 expression (p>0.05). In our study, PMS2 loss was significantly correlated with CDX2 loss (p=0.03). In conclusion, the molecular analysis of biological markers for colon cancer may be important for patient stratification, in order to select the optimal treatment algorithm. Our results suggest that probably the double panel (MSH6 and PMS2) is enough to detect the MSI status, instead of using the quadruple panel.
Databáze: OpenAIRE
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