Synergistic Effect of ACC Deaminase Producing Pseudomonas sp. TR15a and Siderophore Producing Bacillus aerophilus TR15c for Enhanced Growth and Copper Accumulation in Helianthus annuus L
| Autor: | L. Benedict Bruno, Maria Maleva, Adarsh Kumar, Tripti, Mani Rajkumar |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Siderophore
1 AMINOCYCLOPROPANE 1 CARBOXYLATE DEAMINASE GENOMIC DNA MINIMUM INHIBITORY CONCENTRATION 0208 environmental biotechnology ved/biology.organism_classification_rank.species Siderophores PUBLIC RISKS INDOLE-3-ACETIC ACID PROTEIN Bacillus 02 engineering and technology SUNFLOWER SOIL MICROORGANISM 01 natural sciences SIDEROPHORE NUCLEOTIDE SEQUENCE SOIL POLLUTION BACTERIAL DNA CHEMISTRY RNA Ribosomal 16S Soil Pollutants Carbon-Carbon Lyases SYNERGISM Strain (chemistry) DROUGHT RESISTANCE CONCENTRATION (PARAMETER) Pseudomonas BACTERIAL ENZYME BACTERIAL STRAIN General Medicine BIOACCUMULATION BACTERIUM ISOLATION Pollution Bacillus aerophilus INDOLEACETIC ACID PLANT ROOT PHOSPHATE SOLUBILIZATION SOLUBILIZATION WHITE CLOVER GROWTH REGULATOR SHOOT Helianthus PSEUDOMONAS BACILLUS ANTIBIOTICS AEROPHILUS AMPICILLIN MICROBIOLOGY Environmental Engineering SEED KANAMYCIN 16S RRNA GENE SEQUENCING Microbiology CARBON-CARBON LYASES Helianthus annuus NONHUMAN HEAVY METAL Ecosystem BACILLUS AEROPHILUS AMMONIA HEALTH RISKS ved/biology SOIL POLLUTANTS Public Health Environmental and Occupational Health STENOTROPHOMONAS RHIZOPHILA INOCULATION SOIL POLLUTANT 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE LYASE RESISTANCE TRIFOLIUM REPENS SIDEROPHORES Health Toxicology and Mutagenesis METAL TOLERANCE RNA RIBOSOMAL 16S 1 AMINOCYCLOPROPANE 1 CARBOXYLATES COPPER ROOT-SHOOT RATIO 010501 environmental sciences BACTERIAL RNA UNCLASSIFIED DRUG Plant Roots BIOMASS SOIL MICROBIOLOGY PLANTS (BOTANY) GENE SEQUENCE PHOSPHATE Soil Microbiology biology CHEMICAL ANALYSIS RHIZOBIALES Chemistry PSEUDOMONAS SP PLANT GROWTH PROMOTION HELIANTHUS CYANIDE BACTERIA AGRICULTURAL INOCULATION COPPER ACCUMULATION STENOTROPHOMONAS PUBLIC HEALTH PLANT SEED TETRACYCLINE UNINOCULATED CONTROL GERMINATION GENE EXPRESSION GENETICS RNA 16S PENICILLIN DERIVATIVE Rhizobacteria ANTIBIOTIC RESISTANCE Environmental Chemistry PLANT GROWTH PROMOTING ARTICLE BACTERIOLOGY CONSORTIUM OF RHIZOBACTERIA 0105 earth and related environmental sciences STREPTOMYCIN General Chemistry biology.organism_classification HELIANTHUS ANNUUS 020801 environmental engineering CONTROLLED STUDY PLANT GROWTH PLANT ROOTS Trifolium repens RNA ECOSYSTEM CHLORAMPHENICOL HYDROGEN CYANIDE Copper Bacteria |
| Zdroj: | Chemosphere |
| Popis: | Copper (Cu) is an essential element, however it's excess into the environment causes detrimental effect on plant and risks for public health. Four Cu and drought tolerant 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing rhizobacteria were isolated from the roots of Trifolium repens L. growing on Cu smelter contaminated soils, characterized and identified based on 16S rRNA gene sequencing. A consortium of high ACC deaminase (53.74 μM α-ketobutyrate mg−1 protein h−1) producing bacteria Pseudomonas sp. strain TR15a + siderophore producing Bacillus aerophilus strain TR15c significantly (p < 0.05) produced better results for multiple-metal tolerance including Cu (1750 mg kg−1), antibiotic resistance (ampicillin, kanamycin, chloramphenicol, penicillin, tetracycline, and streptomycin) and plant growth promoting attributes (phosphate solubilization: 315 mg L−1, indole-3-acetic acid (IAA) production: 8 mg L−1, ammonia and hydrogen cyanide production) as compared to individual isolates. Pot scale experiment (enriched with 100 mg Cu kg−1) showed inoculation of Helianthus annuus seeds with consortium of TR15a + TR15c had significantly (p < 0.05) improved seed germination by 32%, total dry biomass by 64%, root Cu by 47% and shoot Cu by 75% as compared to uninoculated control whereas 0.2−7 fold higher results were observed for above stated parameters as compared to four individual isolates studied. The result suggests consortium of ACC deaminase producing Pseudomonas sp. TR15a and siderophore producing B. aerophilus TR15c could play a vital role in enhanced Cu uptake and improvement of biomass and may provide a better alternative for decontamination of Cu contaminated natural ecosystem than individual isolates. © 2021 Elsevier Ltd. The authors acknowledge the work support by RFBR, Russia (Project No. 19-516-45006) and DST, India (INT/ RUS / RFBR /363) and the Ministry of Science and Higher Education of the Russian Federation (agreement No. 02.A03.21.0006). We also thank Prof. Katarzyna Turnau and Rafal Wazny, Department of Bioremediation, Malopolska Center of Biotechnology, University of Jagiellonian, Krakow for giving assistance in identification of isolated rhizobacteria. |
| Databáze: | OpenAIRE |
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