Effect of cDMEM media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and differentiation

Autor: Muazzez Gokalp, Elif Sepet, Noushin Zibandeh, Kamber Kasali, Deniz Genç, Damla Tuncer Budanur, Tunc Akkoc
Přispěvatelé: Tuncer Budanur D., Zibandeh N., Genc D., Gokalp M., KAŞALİ K., AKKOÇ T., Sepet E.
Rok vydání: 2018
Předmět:
0301 basic medicine
Necrosis
Vimentin
Ectoine
Sağlık Bilimleri
Clinical Medicine (MED)
chemistry.chemical_compound
0302 clinical medicine
Diş Hekimliği
Ortodonti
Klinik Tıp (MED)
PLASTICITY
GROWTH-FACTORS
biology
Klinik Tıp
Chemistry
DENTISTRY
ORAL SURGERY & MEDICINE
Cell Differentiation
Flow Cytometry
Tıp
periodontal ligament cells
Milk
Osmoregulation
Dental Hygiene
Medicine
Periodontics
medicine.symptom
Oral Surgery
Adult
Dental Assisting
Adolescent
Cell Survival
Periodontal Ligament
Diş Hekimliği (çeşitli)
osteogenic differentiation
Orthodontics
Diş Hijyeni
COCONUT WATER
03 medical and health sciences
ectoine
AVULSED TEETH
Health Sciences
medicine
Periodontal fiber
Animals
Humans
Dentistry (miscellaneous)
Viability assay
General Dentistry
cell viability
fibroblastic differentiation
STORAGE MEDIA
Mesenchymal stem cell
Periodontoloji
Ağız Cerrahisi
Amino Acids
Diamino
Water
Mesenchymal Stem Cells
030206 dentistry
IN-VITRO
CLINICAL MEDICINE
Molecular biology
TRAUMATIC DENTAL INJURIES
VIABILITY
Culture Media
DİŞ HEKİMLİĞİ
ORAL CERRAHİ VE TIP
030104 developmental biology
Apoptosis
STROMAL CELLS
Dentistry
biology.protein
Dişçilik Hizmetleri
Zdroj: Dental traumatology : official publication of International Association for Dental Traumatology. 34(3)
ISSN: 1600-9657
Popis: Background/Aim: Ectoine is an amino acid that can preserve osmotic balance and has more preservative activity than other osmoregulators. There are no published reports on the osmoregulators’ effects on viability or differentiation of dental stem cells. The aim of this study was to investigate the effect of Ectoine as a storage media on the viability and differentiation potential of human periodontal ligament mesenchymal stem cells (hPDLMSCs). Materials and Methods: hPDLMSCs were obtained from impacted third molar teeth. The cells were isolated, submitted to trilineage differentiation, and characterized by flow cytometer (FC). hPDLMSCs were incubated with different media containing Ectoine, complete DMEM (cDMEM), Ectoine+cDMEM, milk, and tap water for 2, 6, 12, and 24 h. The cells were analyzed by FC for viability, early-apoptosis, late apoptosis, and necrosis rates. Osteogenic and fibroblastic differentiation in hPDLMSCs were investigated by Alizarin red stain and vimentin expression. Results: All treated groups showed significant decreases in cell viability after 2 h. Significant increases were detected in the number of dead cells between 2 and 12 h in all groups except the Ectoine+cDMEM group. The deposition of mineral matrix nodules was significantly higher in cells cultured with Ectoine+cDMEM compared with the other media. Higher vimentin expressions were detected in cells cultured with cDMEM and Ectoine+cDMEM media, respectively. Conclusions: Ectoine added to cDMEM media, promoted cell survival plus osteogenic and fibroblastic differentiation of hPDLMSCs.
Databáze: OpenAIRE
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