Integrated Experimental and Modeling Study of Enzymatic Degradation Using Novel Autofluorescent BSA Microspheres
| Autor: | Ji-Qin Li, Christopher O'Connell, Xiaoyu Ma, Yu Lei, Tai-Hsi Fan |
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| Rok vydání: | 2017 |
| Předmět: |
Models
Molecular Tris Fluorophore Protein Conformation Kinetics Nanoparticle 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Electrochemistry Animals General Materials Science Bovine serum albumin Spectroscopy Fluorescent Dyes Aqueous solution biology Hydrogels Serum Albumin Bovine Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics Proteinase K Microspheres 0104 chemical sciences chemistry Glutaral Proteolysis Biophysics biology.protein Cattle Glutaraldehyde Endopeptidase K 0210 nano-technology |
| Zdroj: | Langmuir. 34:191-197 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | Autofluorescent bovine serum albumin (BSA) hydrogel microspheres were prepared through the spray-drying of glutaraldehyde cross-linked BSA nanoparticles and then used for a proteinase K based degradation study in an aqueous solution. Experimental results and empirical models are presented to characterize the kinetics of BSA hydrogel microsphere degradation, as well as the accompanying release of synthesized fluorophore. The BSA gel degradation dynamics is primarily controlled by the concentration of proteinase K within the Tris buffer. The coupling of swelling dynamics and the transient distributions of fluorophore are traced by confocal microscopy. Models are developed based on the linear theory of elastic deformation coupled to enzyme and fluorophore transport. This study represents a fundamental investigation of the degradation and release kinetics of protein-based materials, which can potentially be applied for the dynamic and photostable tracking of relevant in vivo systems. |
| Databáze: | OpenAIRE |
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