Bovine Herpesvirus 1-Induced Apoptotic Cell Death: Role of Glycoprotein D
| Autor: | Paul-Pierre Pastoret, Lorne A. Babiuk, Philip J. Griebel, Alain Vanderplasschen, Günther M. Keil, Sylvia van Drunen Littel-van den Hurk, Emmanuel Hanon, Frans A.M. Rijsewijk |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.drug_class
animal diseases viruses Immunoglobulins Apoptosis Monoclonal antibody Viral Proteins Viral envelope Antigen Virology Tumor Cells Cultured medicine Life Science Animals Instituut voor Dierhouderij en Diergezondheid Antigens Viral Herpesvirus 1 Bovine chemistry.chemical_classification ID-Lelystad biology Antibodies Monoclonal virus diseases biochemical phenomena metabolism and nutrition Flow Cytometry biology.organism_classification Molecular biology Herpesvirus glycoprotein B Bovine herpesvirus 1 ID Lelystad chemistry ID-Lelystad Instituut voor Dierhouderij en Diergezondheid ID Lelystad Institute for Animal Science and Health biology.protein Cattle Antibody Glycoprotein Institute for Animal Science and Health |
| Zdroj: | Virology, 257, 191-197 Virology 257 (1999) |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1999.9620 |
| Popis: | Bovine herpesvirus 1 (BHV-1) induces apoptotic cell death in peripheral blood mononuclear cells and in bovine B lymphoma (BL-3) cells. Attachment but not penetration of BHV-1 is necessary to induce apoptosis in target cells, suggesting that one or more BHV-1 envelope glycoproteins could be involved in the activation of the apoptotic process. In the present study, we demonstrate that, although BHV-1 virions devoid of glycoprotein D (BHV-1 gD−/−) still bind to BL-3 cells, they are no longer able to induce apoptosis. In contrast, virions that contain glycoprotein D (gD) in the viral envelope but do not genetically encode gD (BHV-1 gD−/+) induce a level of apoptosis comparable to that produced by wild-type (wt) BHV-1. In addition, monoclonal antibodies directed against gD, but not against gB or gC, strongly reduced the high levels of apoptosis induced by BHV-1. These observations demonstrate that the induction of apoptosis is directly due to BHV-1 viral particles harboring gD in the viral envelope. Interestingly, binding of affinity-purified gD to BL-3 cells did not induce apoptosis but inhibited the ability of wt BHV-1 to induce apoptosis. Altogether, these results provide evidence for the direct or indirect involvement of gD in the mechanism by which BHV-1 induces apoptosis. |
| Databáze: | OpenAIRE |
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