Adipose-specific Lipoprotein Lipase Deficiency More Profoundly Affects Brown than White Fat Biology
| Autor: | Sheila M. O'Byrne, William S. Blaner, Manabu Takahashi, Konstantinos Drosatos, Yunying Hu, Ni Huiping Son, Ira J. Goldberg, Itsaso Garcia-Arcos, Yaeko Hiyama, Chuchun L. Chang, Richard J. Deckelbaum, Joseph C. Obunike, Kalyani G. Bharadwaj, Marit Westerterp, Hongfeng Jiang, Hiroaki Yagyu |
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| Přispěvatelé: | Center for Liver, Digestive and Metabolic Diseases (CLDM), Translational Immunology Groningen (TRIGR), Medical Biochemistry |
| Rok vydání: | 2013 |
| Předmět: |
Adipose Tissue/metabolism
Male Adipose Tissue Brown/metabolism Adipose tissue White adipose tissue Biochemistry Mice chemistry.chemical_compound Adipose Tissue Brown Adipocyte Chylomicrons Brown adipose tissue Adipocytes Bone Marrow Transplantation Mice Knockout Lipoprotein lipase Adipocytes/cytology digestive oral and skin physiology Lipids White/metabolism Triglycerides/blood Phenotype medicine.anatomical_structure Adipose Tissue lipids (amino acids peptides and proteins) medicine.medical_specialty Chylomicrons/pharmacokinetics Adipose Tissue White/metabolism Knockout Adipose Tissue White Lipolysis Lipids/chemistry Biology Brown/metabolism Internal medicine medicine Animals Molecular Biology Triglycerides Triglyceride Macrophages Macrophages/cytology nutritional and metabolic diseases Cell Biology Lipoprotein Lipase/deficiency Lipoprotein Lipase Endocrinology chemistry Chylomicron |
| Zdroj: | The Journal of Biological Chemistry, 288(20), 14046-14058. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC Journal of biological chemistry, 288(20), 14046-14058. American Society for Biochemistry and Molecular Biology Inc. |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m113.469270 |
| Popis: | Adipose fat storage is thought to require uptake of circulating triglyceride (TG)-derived fatty acids via lipoprotein lipase (LpL). To determine how LpL affects the biology of adipose tissue, we created adipose-specific LpL knock-out (ATLO) mice, and we compared them with whole body LpL knock-out mice rescued with muscle LpL expression (MCK/L0) and wild type (WT) mice. ATLO LpL mRNA and activity were reduced, respectively, 75 and 70% in gonadal adipose tissue (GAT), 90 and 80% in subcutaneous tissue, and 84 and 85% in brown adipose tissue (BAT). ATLO mice had increased plasma TG levels associated with reduced chylomicron TG uptake into BAT and lung. ATLO BAT, but not GAT, had altered TG composition. GAT from MCK/L0 was smaller and contained less polyunsaturated fatty acids in TG, although GAT from ATLO was normal unless LpL was overexpressed in muscle. High fat diet feeding led to less adipose in MCK/L0 mice but TG acyl composition in subcutaneous tissue and BAT reverted to that of WT. Therefore, adipocyte LpL in BAT modulates plasma lipoprotein clearance, and the greater metabolic activity of this depot makes its lipid composition more dependent on LpL-mediated uptake. Loss of adipose LpL reduces fat accumulation only if accompanied by greater LpL activity in muscle. These data support the role of LpL as the "gatekeeper" for tissue lipid distribution. |
| Databáze: | OpenAIRE |
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