Effects of cerulenin on the endogenous fatty acid synthetic activity in squamous cell carcinoma of the oral cavity
| Autor: | Nian-hui Cui, Ding-xin Liu, Qing Song, Chuan-Bin Guo, Shu-cong Meng, Guang-Yan Yu |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Time Factors Cell Endogeny Pharmacology Acetic acid chemistry.chemical_compound Culture Techniques Medicine Humans Basal cell Dimethyl Sulfoxide Carbon Radioisotopes Oral mucosa Enzyme Inhibitors Fatty acid synthesis Aged chemistry.chemical_classification Analysis of Variance business.industry Fatty Acids Mouth Mucosa Fatty acid Middle Aged Cerulenin stomatognathic diseases medicine.anatomical_structure Otorhinolaryngology chemistry Immunology Carcinoma Squamous Cell Solvents lipids (amino acids peptides and proteins) Surgery Female Mouth Neoplasms Oral Surgery Fatty Acid Synthases Radiopharmaceuticals business |
| Zdroj: | Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons. 61(8) |
| ISSN: | 0278-2391 |
| Popis: | Purposes: Inhibition of cerulenin on the endogenous fatty acid synthetic activities of oral squamous cell carcinoma (OSCC) and normal oral mucosa was assayed. Methods: Squamous cell carcinoma and normal oral mucosa were collected fresh from surgical specimens. The collected tissues were minced in RPMI 1640 and divided into 3 groups: cerulenin treated, dimethylsulfoxide treated, and control. The tissues were incubated in [1(2)- 14 C]acetic acid, sodium salt for the last 2.5 hours of the treatment at 37°C in 5% CO 2 . After labeling, total lipids were extracted and counted for 14 C by scintillation counting. Results: Endogenous fatty acid synthetic activities of oral squamous cell caranoma in the cerulenin-treated group decreased by 19% at 1 hour, 64% at 2 hours, and 87% at 4 hours; remained nearly unchanged in the dimethylsulfoxide-treated group; and increased slightly in the control group. The oral mucosa tissues were only mildly affected by cerulenin in fatty acid synthesis. Conclusions: Cerulenin significantly inhibits fatty acid synthetic activity in squamous cell carcinoma and only mildly affected the oral mucosa, indicating that the fatty acid synthetic pathway may be exploited as a target for developing anticancer drugs. |
| Databáze: | OpenAIRE |
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