c-myc, ras p21 and p53 Expression in pleomorphic adenoma and its malignant form of the human salivary glands
| Autor: | Hironori Hamano, Yoshio Hayashi, Hiroyo Deguchi |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adult
Pathology medicine.medical_specialty endocrine system diseases Immunoblotting Adenoma Pleomorphic Genes myc Gene Expression Biology Salivary Glands Pathology and Forensic Medicine Malignant transformation Immunoenzyme Techniques Proto-Oncogene Proteins c-myc Proto-Oncogene Proteins p21(ras) Pleomorphic adenoma medicine Carcinoma Humans P53 expression Aged Salivary gland Significant difference Salivary Gland Pleomorphic Adenoma General Medicine Middle Aged Genes p53 Salivary Gland Neoplasms medicine.disease digestive system diseases stomatognathic diseases Cell Transformation Neoplastic medicine.anatomical_structure Cancer research Immunohistochemistry |
| Zdroj: | Pathology International. 43:413-422 |
| ISSN: | 1440-1827 1320-5463 |
| Popis: | Using an immunohistochemical study and an immunoblot analysis, the expression of cellular oncogenes of the human salivary glands such as c-myc, ras p21, and p53 tumor-suppressor gene in pleomorphic adenomas and its malignant form, carcinoma in pleomorphic adenomas was examined to evaluate a differential biological significance, in comparison with that in normal salivary gland tissues. Immunohistochemically, the c-myc product was detected in 42% of the pleomorphic adenomas and in 56% of the carcinomas in pleomorphic adenoma. The ras p21 expression was observed in 24% of pleomorphic adenomas, and in 50% of carcinomas in pleomorphic adenoma. The p53 protein was detected in 18% of the pleomorphic adenomas and in 67% of the carcinomas in pleomorphic adenoma. Although there was no significant difference between the benign and malignant forms for the expression of c-myc, a statistical significance in ras p21 and p53 expression was found between the pleomorphic adenoma and its malignant form (P < 0.05) and P < 0.001, respectively). An immunoblotting assay clearly demonstrated the expression of c-myc and p53 gene products in both the benign and malignant forms of the pleomorphic adenoma, and that of ras p21 in the malignant form. These results indicate that activation of c-myc and ras p21 proto-oncogenes and the involvement of p53 mutation may play important roles in the malignant transformation of salivary gland pleomorphic adenoma. |
| Databáze: | OpenAIRE |
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