Expression pattern of cellulolytic and xylanolytic genes regulated by transcriptional factors XYR1 and CRE1 are affected by carbon source in Trichoderma reesei
| Autor: | Rafael Silva-Rocha, Gabriela F. Persinoti, Roberto Nascimento Silva, Amanda Cristina Campos Antoniêto, Wellington Ramos Pedersoli, Lilian dos Santos Castro |
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| Rok vydání: | 2014 |
| Předmět: |
Sophorose
Mutant Cellulase Microbiology Fungal Proteins chemistry.chemical_compound Gene Expression Regulation Fungal Gene expression Genetics Promoter Regions Genetic Molecular Biology Gene Trichoderma reesei Trichoderma Binding Sites biology Gene Expression Profiling Promoter biology.organism_classification Carbon chemistry Biochemistry biology.protein Xylanase Carbohydrate Metabolism TRICHODERMA Protein Binding Transcription Factors Developmental Biology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1567-133X |
| DOI: | 10.1016/j.gep.2014.01.003 |
| Popis: | Trichoderma reesei is the most important fungus for the industrial production of enzymes to biomass deconstruction. Most of the genes encoding cellulases and hemicellulases are regulated by the transcription factors CRE1 and XYR1. In this work, the regulation of 22 genes of cellulases and xylanases by these transcription factors was investigated under three different carbon sources. Analysis of gene expression and enzymatic profiles of CMCase, β-glucosidase, and xylanases showed different regulation that was depended of the carbon source in both Δxyr1 and Δcre1 mutants. In the presence of glucose, the majority of genes evaluated (82%) showed increased expression levels in the Δcre1 mutant compared to the parental QM9414 strain. In the Δxyr1 mutant, it was observed that expression of cellulase and xylanase genes was reduced compared to the parental QM9414 strain, when cultured in the presence of cellulose or sophorose. Interesting, in the presence of glucose, approximately 60% of the analyzed genes had increased expression in the Δxyr1 mutant compared to parental strain. Furthermore, no correlation between gene expression and the number of putative binding sites of XYR1 and CRE1 to promoter region of cellulolytic and xylanolytic studied genes was observed. Therefore, these results demonstrated that the regulation of cellulase and xylanase by the transcription factors CRE1 and XYR1 is influenced by different carbon sources. |
| Databáze: | OpenAIRE |
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