Determination of cucurbitacin E in some selected herbs of ayurvedic importance through RP-HPLC
| Autor: | Joydeb Chanda, Sayan Biswas, Pulok K. Mukherjee, Amit Kar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0211 other engineering and technologies
02 engineering and technology 03 medical and health sciences Cucurbita pepo chemistry.chemical_compound 0302 clinical medicine Nutraceutical Validation 021105 building & construction Drug Discovery Hplc method Mathematics Cucurbitacin E Traditional medicine biology Lagenaria Repeatability biology.organism_classification Ich guideline Standardization 030205 complementary & alternative medicine Cucurbitaceae Complementary and alternative medicine chemistry Original Research Article (Experimental) RP-HPLC Ayurveda |
| Zdroj: | Journal of Ayurveda and Integrative Medicine |
| ISSN: | 0975-9476 |
| Popis: | Background The consumption of the fruits of cucurbitaceae plants is widely popular among Indians due to their various nutritional and medicinal purposes. Some of these plants are well reported in Ayurveda due to their potential therapeutic importance. In particular, the plants of this family are well-characterized by the presence of its bitter principle, Cucurbitacin E which differs within the species due to its genetic variations. Objectives The objective of the study was to develop a validated RP-HPLC method for standardization in some widely consumed cucurbits with cucurbitacin E as a marker compound. Materials and methods The RP-HPLC method was developed with a reverse phase C18 column, using acetonitrile and water (1% glacial acetic acid) as mobile phase (70:30 v/v). The flow rate and λmax were optimized at 1 mL/min and 230 nm respectively. The HPLC method was validated in terms of accuracy, specificity, sensitivity, and repeatability as per ICH guideline. Results The calibration curve was found linear in the concentration range of 1–100 μg/mL. The % RSD of precision and recovery was found to be Graphical abstract Image 1 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|