Therapeutic tissue regenerative nanohybrids self-assembled from bioactive inorganic core / chitosan shell nanounits
| Autor: | Jeong-Hui Park, Nandin Mandakhbayar, Sung-Jin Kim, Hae-Won Kim, Ji-Young Yoon, Jun-Hyeog Jang, Rajendra K. Singh, Jonathan C. Knowles, Ueon Sang Shin, Guang-Zhen Jin, Seung Bin Jo, Jung-Hwan Lee, Han-Sem Kim |
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| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Biophysics Nanoparticle Bioengineering Core (manufacturing) Nanotechnology 02 engineering and technology law.invention Self assembled Biomaterials Chitosan 03 medical and health sciences chemistry.chemical_compound law Osteogenesis 030304 developmental biology 0303 health sciences Mesoporous silica 021001 nanoscience & nanotechnology Silicon Dioxide Durapatite chemistry Mechanics of Materials Bioactive glass Ceramics and Composites Nanoparticles Self-assembly 0210 nano-technology |
| Zdroj: | Biomaterials. 274 |
| ISSN: | 1878-5905 |
| Popis: | Natural inorganic/organic nanohybrids are a fascinating model in biomaterials design due to their ultra-microstructure and extraordinary properties. Here, we report unique-structured nanohybrids through self-assembly of biomedical inorganic/organic nanounits, composed of bioactive inorganic nanoparticle core (hydroxyapatite, bioactive glass, or mesoporous silica) and chitosan shell - namely Chit@IOC. The inorganic core thin-shelled with chitosan could constitute as high as 90%, strikingly contrasted with the conventional composites. The Chit@IOC nanohybrids were highly resilient under cyclic load and resisted external stress almost an order of magnitude effectively than the conventional composites. The nanohybrids, with the nano-roughened surface topography, could accelerate the cellular responses through stimulated integrin-mediated focal adhesions. The nanohybrids were also able to load multiple therapeutic molecules in the core and shell compartment and then release sequentially, demonstrating controlled delivery systems. The nanohybrids compartmentally-loaded with therapeutic molecules (dexamethasone, fibroblast growth factor 2, and phenamil) were shown to stimulate the anti-inflammatory, pro-angiogenic and osteogenic events of relevant cells. When implanted in the in vivo calvarium defect model with 3D-printed scaffold forms, the therapeutic nanohybrids were proven to accelerate new bone formation. Overall, the nanohybrids self-assembled from Chit@IOC nanounits, with their unique properties (ultrahigh inorganic content, nano-topography, high resilience, multiple-therapeutics delivery, and cellular activation), can be considered as promising 3D tissue regenerative platforms. |
| Databáze: | OpenAIRE |
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