Chronic valproate treatment influences folliculogenesis and reproductive hormones with possible ameliorating role for folic acid in adult albino rats
| Autor: | Adel Mohamed Aboregela, Rehab Hassan Elbanna Gouda, Khaled Adel Eid, Ibrahim Hassan Ibrahim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Histology Sodium chemistry.chemical_element Ovary Transforming Growth Factor beta1 03 medical and health sciences 0302 clinical medicine Folic Acid Ovarian Follicle Internal medicine Proliferating Cell Nuclear Antigen Medicine Animals Rats Wistar Gonadal Steroid Hormones Testosterone biology business.industry Valproic Acid Cell Biology General Medicine Proliferating cell nuclear antigen Rats 030104 developmental biology Endocrinology medicine.anatomical_structure Folic acid chemistry 030220 oncology & carcinogenesis biology.protein Immunohistochemistry Female Folliculogenesis business |
| Zdroj: | Acta histochemica. 121(7) |
| ISSN: | 1618-0372 |
| Popis: | Sodium Valproate (VPA) is known to have deleterious consequences on ovarian function and folliculogenesis. Folic acid (FA) is associated with the quality of many parameters in folliculogenesis. Therefore, we aimed to investigate the effects of chronic Valproate administration on ovarian morphology, folliculogenesis, reproductive hormones, and the possible protective effect of Folic acid supplementation. Forty adult female albino rats were divided into four groups and treated orally for 90 days as follows: Control group received distilled water; FA group received (folic acid 400 μg/day); VPA group received (Na Valproate 200 mg/kg/day) and VPA + FA group received (Na Valproate 200 mg/kg/day + folic acid 400 μg/day). In addition, ovaries were processed for routine histology and immunohistochemistry (TGFβ1 and PCNA) and reproductive hormones levels were measured. Results showed a significant decrease in number of follicles in VPA group, while atretic follicles increased compared with control group (P 0.001). Interestingly, the number of follicles significantly increased in VPA + FA group compared with VPA group (P 0.001). Also, number of atretic follicles significantly decreased in the VPA + FA group compared to the VPA group. Histochemistry score decreased for TGFβ1 and PCNA staining in VPA group compared with control group (P 0.01). Moreover, Valproate demonstrated a significant increase in testosterone levels in VPA group than control group (P 0.001). However, VPA group demonstrated a significant decrease in levels of estradiol, progesterone, FSH and LH levels compared with control group. These changes were partially improved in VPA + FA group. In conclusion, FA co-treatment can modulate ovarian follicular and hormonal disturbances induced by valproate, which needs further investigations to identify the precise mechanisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect