Effect of berberine on proliferation, biosynthesis of macromolecules, cell cycle and induction of intercalation with DNA, dsDNA damage and apoptosis in Ehrlich ascites carcinoma cells
| Autor: | Mira Horvathova, Milan Miko, Silvia Letasiova, Soňa Jantová, Renáta Ovádeková |
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| Rok vydání: | 2006 |
| Předmět: |
Berberine
DNA damage Pharmaceutical Science Apoptosis Biology Ehrlich ascites carcinoma chemistry.chemical_compound Tumor Cells Cultured Animals RNA Neoplasm Cytotoxicity Carcinoma Ehrlich Tumor Cell Proliferation Pharmacology DNA synthesis Cell Cycle Proteins DNA DNA Neoplasm Cell cycle Molecular biology chemistry Growth inhibition DNA Damage |
| Zdroj: | The Journal of pharmacy and pharmacology. 58(2) |
| ISSN: | 0022-3573 |
| Popis: | Our primary aim was to study berberine, a potential anti-cancer drug, for its cytotoxic and antiproliferative activity in-vitro using Ehrlich ascites carcinoma (EAC) cells. Cytotoxicity was measured by the growth inhibition assay. We investigated the effect of berberine on the biosynthesis of macro-molecules (DNA, RNA, proteins), cell cycle effects and induction of dsDNA damage and apoptosis in berberine-treated EAC cells. Our results showed that berberine acts cytotoxically on EAC cells. The cytotoxicity was directly concentration and time dependent. The highest cytotoxic concentrations (100 and 50 μg mL−1) induced intercalation of berberine with DNA, formation of dsDNA breaks, inhibition of DNA synthesis and death of EAC cells. A concentration of 10 μg mL−1 induced clear apoptotic cell death, which was followed by inhibition of protein synthesis. |
| Databáze: | OpenAIRE |
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