Distribution of DNA replicator sites in mammalian nuclei after different methods of cell synchronization
| Autor: | Catherine A. Williams, C.H. Ockey |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
DNA Replication
Mitosis Biology Tritium Cell Line Amethopterin chemistry.chemical_compound Cricetinae Culture Techniques Methods medicine Animals Cell synchronization Cell Nucleus DNA synthesis Ovary DNA replication DNA Cell Biology Fibroblasts Molecular biology Cell biology Chromatin Microscopy Electron Methotrexate medicine.anatomical_structure chemistry Autoradiography Female Floxuridine Thymidine Nucleus |
| Zdroj: | Experimental Cell Research. 63:365-372 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(70)90224-7 |
| Popis: | Chinese hamster fibroblasts synchronized by metaphase selection, amethopterin or 5-fluorodeoxyuridine were pulsed labelled in early or late stages of DNA synthesis with 3H-thymidine. Label distributions were studied in EM sections and under light microscopy of flattened nuclei. 1. 1. Early DNA synthesis occurred in replicons evenly distributed over the nucleus. 2. 2. Late DNA synthesis was restricted preferentially to replicons on the periphery of the nucleus. 3. 3. No shift of label occurred during chase periods with unlabelled thymidine. 4. 4. No significant differences between label distributions at the various stages could be found following different synchronization methods. 5. 5. The peripheral labelling during late DNA synthesis reflects synthesis in DNA usually present as condensed chromatin on the nuclear membrane. |
| Databáze: | OpenAIRE |
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