Screening of Plant Extracts for Antioxidant Activity: a Comparative Study on Three Testing Methods

Autor: I. Koleva, Aede de Groot, Teris A. van Beek, Lyuba N. Evstatieva, Jozef P. H. Linssen
Jazyk: angličtina
Rok vydání: 2002
Předmět:
Antioxidant
Time Factors
DPPH
medicine.medical_treatment
Plant Science
Biochemistry
oxidative stability
Antioxidants
Analytical Chemistry
chemistry.chemical_compound
methyl linoleate
vegetable-oils
Drug Discovery
Butylated hydroxytoluene
natural antioxidants
biology
Food Chemistry
Rosmarinic acid
Methyl linoleate
General Medicine
Free Radical Scavengers
Reference Standards
beta Carotene
Organische Chemie
phenolic-acids
beta-carotene
Sideritis
Molecular Medicine
radical-scavenging activity
Chromatography
Gas
Free Radicals
Sensitivity and Specificity
Picrates
beta-Carotene
alpha-tocopherol
tea aspalathus-linearis
Levensmiddelenchemie
medicine
VLAG
performance liquid-chromatography
Chromatography
Lamiaceae
Plants
Medicinal
Plant Extracts
Biphenyl Compounds
Organic Chemistry
biology.organism_classification
Complementary and alternative medicine
chemistry
Gas chromatography
Food Science
Zdroj: Phytochemical Analysis, 13(1), 8-17
Phytochemical Analysis 13 (2002) 1
ISSN: 0958-0344
Popis: Three methods widely employed in the evaluation of antioxidant activity, namely 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS-GC) and β-carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts. The strengths and limitations of each method have been illustrated by testing a number of extracts, of differing polarity, from plants of the genus Sideritis, and two known antioxidants (butylated hydroxytoluene and rosmarinic acid). The sample polarity was important for the exhibited activity in the BCBT and HS-GC methods but not for the DPPH method. The complex composition of the extracts and partition phenomena affected their activity in each assay. The value of the BCBT method appears to be limited to less polar samples. Although slow, the HS-GC method is preferable for assessing the antioxidant inhibitory properties on the formation of unwanted secondary volatile products. Being rapid, simple and independent of sample polarity, the DPPH method is very convenient for the quick screening of many samples for radical scavenging activity. Copyright © 2001 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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