Enhanced production of questin by marine-derived Aspergillus flavipes HN4-13
| Autor: | Xiaowen Xu, Fuxuan Xu, Jiacai Guo, Lei Guo, Le Wang, Weiqin Yang, Fuhou Li, Xiangrong Li, Xintong Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Chemistry
Starch Significant difference Fractional factorial design Environmental Science (miscellaneous) Agricultural and Biological Sciences (miscellaneous) law.invention ASPERGILLUS FLAVIPES chemistry.chemical_compound Erlenmeyer flask Short Reports law Fermentation Food science Response surface methodology Beef extract Biotechnology |
| Zdroj: | 3 Biotech |
| ISSN: | 2190-5738 2190-572X |
| Popis: | Questin has favorable applications. Fractional factorial design, Box–Behnken design, and response surface methodology were adopted to optimize the fermentation conditions of the marine-derived fungus, Aspergillus flavipes HN4-13, thereby enhancing questin production. Optimal fermentation conditions in a 500-mL conical flask with 200 mL of medium were 4% soluble starch, 0.9% beef extract, 4% NaCl, 0.05% Na(2)HPO(4), pH 6, 2% inoculum size, and shaking at 28 ℃ and 160 rpm/min for 7 days. The production of questin can achieve 64.93 ± 4.55 mg/L, with no significant difference from the predicted value (66.27 mg/L). Thus, this optimized process of questin production is feasible. Such production is 17-fold higher than that of the basal Sabouraud’s dextrose medium. Results indicate the potential of A. flavipes HN4-13 in the large-scale production of questin through fermentation. |
| Databáze: | OpenAIRE |
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