The relationship between microbial community structure and functional stability, tested experimentally in an upland pasture soil
| Autor: | Lesley Anne Glover, C. Fenwick, H. L. Kuan, Allison E. McCaig, Bryan S. Griffiths, Karl Ritz |
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| Rok vydání: | 2004 |
| Předmět: |
Hot Temperature
Fumigation Soil Science Biology Bacterial Physiological Phenomena complex mixtures Pasture Microbial ecology Ecosystem Ecology Evolution Behavior and Systematics Soil Microbiology Biomass (ecology) geography Analysis of Variance Principal Component Analysis geography.geographical_feature_category Nitrates Ecology Bacteria Reverse Transcriptase Polymerase Chain Reaction Carbon Dioxide Quaternary Ammonium Compounds Agronomy Microbial population biology Scotland Gamma Rays Soil water Chloroform Soil microbiology Copper |
| Zdroj: | Microbial ecology. 47(1) |
| ISSN: | 0095-3628 |
| Popis: | Soil collected from an upland pasture was manipulated experimentally in ways shown previously to alter microbial community structure. One set of soil was subjected to chloroform fumigation for 0, 0.5, 2, or 24 h and the other was sterilised by gamma-irradiation and inoculated with a 10(-2), 10(-4), 10(-6), or 10(-8) dilution of a soil suspension prepared from unsterilized soil. Following incubation for 8 months, to allow for the stabilization of microbial biomass and activity, the resulting microbial community structure (determined by PCR-DGGE of bacterial specific amplification products of total soil DNA) was assessed. In addition, the functional stability (defined here as the resistance and resilience of short-term decomposition of plant residues to a transient heat or a persistent copper perturbation) was determined. Changes in the active bacterial population following perturbation (determined by RT-PCR-DGGE of total soil RNA) were also monitored. The manipulations resulted in distinct shifts in microbial community structure as shown by PCR-DGGE profiles, but no significant decreases in the number of bands. These shifts in microbial community structure were associated with a reduction in functional stability. The clear correlation between altered microbial community structure and functional stability observed in this upland pasture soil was not evident when the same protocols were applied to soils in other studies. RT-PCR-DGGE profiles only detected a shift in the active bacterial population following heat, but not copper, perturbation. We conclude that the functional stability of decomposition is related to specific components of the microbial community. |
| Databáze: | OpenAIRE |
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