Mass spectrometric analysis of adducts of sulfur mustard analogues to human plasma proteins: approach towards chemical provenancing in biomedical samples

Autor:	Debora van der Riet-van Oeveren, A. Fidder, Maria Hemme, Daan Noort, Marcel J. van der Schans
Rok vydání:	2021
Předmět:	02 engineering and technology Tripeptide Pronase 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry Adduct chemistry.chemical_compound Mustard Gas Humans Chemical Warfare Agents Histidine Primary (chemistry) Chromatography biology 010401 analytical chemistry Sulfur mustard Blood Proteins 021001 nanoscience & nanotechnology Proteinase K Blood proteins 0104 chemical sciences chemistry biology.protein 0210 nano-technology Biomarkers
Zdroj:	Analytical and Bioanalytical Chemistry. 413:4023-4036
ISSN:	1618-2650 1618-2642
DOI:	10.1007/s00216-021-03354-z
Popis:	The primary aim of this study was to identify biomarkers of exposure to some so-called Schedule 1 sulfur mustard (HD) analogues, in order to facilitate and expedite their retrospective analysis in case of alleged use of such compounds. Since these HD analogues can be regarded as model compounds for possible impurities of HD formed during synthesis processes, the secondary aim was to explore to which extent these biomarkers can be used for chemical provenancing of HD in case biomedical samples are available. While the use of chemical attribution signatures (CAS) for neat chemicals or for environmental samples has been addressed quite frequently, the use of CAS for investigating impurities in biomedical samples has been addressed only scarcely. Human plasma was exposed to each of the five HD analogues. After pronase or proteinase K digestion of precipitated protein and sample work-up, the histidine (His) and tripeptide (CPF) adducts to proteins were analyzed, respectively. Adducts of the analogues could still be unambiguously identified next to the main HD adducts in processed plasma samples after exposure to HD mixed with each of the analogues, at a 1% level relative to HD. In conclusion, we have identified plasma protein adducts of a number of HD analogues, which can be used as biomarkers to assess an exposure to these Schedule 1 chemicals. We have shown that adducts of these analogues can still be analyzed after work-up of plasma samples which had been exposed to these analogues in a mixture with HD, supporting the hypothesis that biomedical sample analysis might be useful for chemical provenancing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e27b83e47064deee2378f9ddf5d40481 https://doi.org/10.1007/s00216-021-03354-z Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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