Cyclic mechanical stretching and interleukin-1alpha synergistically up-regulate prostacyclin secretion in cultured human uterine myometrial cells
| Autor: | Norimasa Sagawa, Daizo Korita, Masahiro Yoshida, Shigeo Yura, Mercy A. Nuamah, S. Fujii, Kazuyo Kakui, Maki Takemura, Hiroshi Itoh |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Periodicity Endocrinology Diabetes and Metabolism Prostaglandin Prostacyclin Prostacyclin secretion 6-Ketoprostaglandin F1 alpha Dinoprost Mechanical stretching Mechanotransduction Cellular Dinoprostone Muscle Smooth Vascular Umbilical Arteries chemistry.chemical_compound Thromboxane A2 Endocrinology Internal medicine medicine Humans Secretion Prostaglandin E2 Cells Cultured Obstetrics and Gynecology Coronary Vessels Epoprostenol Biomechanical Phenomena Thromboxane B2 chemistry Interleukin 1α Myometrium lipids (amino acids peptides and proteins) Female medicine.drug Interleukin-1 |
| Zdroj: | Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology. 18(3) |
| ISSN: | 0951-3590 |
| Popis: | Prostacyclin (PGI2), a potent uterine smooth muscle relaxant, is postulated to be a major prostaglandin (PG) secreted from the human myometrium. PGI2 metabolite concentrations in the maternal plasma were reported to be elevated during pregnancy, especially during labor. Recently, we developed cultured human myometrial cells from pregnant women and reported that cyclic mechanical stretching mimicking labor increased PGI2 secretion from these cells by up-regulating PGI2 synthase promoter activities. Since elevation of cervical/vaginal interleukin-1alpha (IL-1alpha) concentrations is also a characteristic feature of delivery, and IL-1alpha is a known stimulator of PG synthesis, we investigated a possible synergistic effect of cyclic mechanical stretching and IL-1alpha on PGI2 production in cultured human myometrial cells. Treatment with IL-1alpha (10 ng/ml) significantly augmented (4- to 60-fold) the secretion of PGI2, prostaglandin E2 (PGE2), prostaglandin F2alpha (PGF2alpha) and thromboxane A2 (TXA2) from cultured human myometrial cells obtained from non-pregnant and pregnant women as well as in cultured human umbilical artery and cultured human coronary artery smooth muscle cells (p0.05 for all comparisons). However, labor-like cyclic mechanical stretching up-regulated IL-1alpha-augmented PGI2 secretion from myometrial cells obtained from non-pregnant and pregnant women 2.1- to 2.8-fold (p0.05 for all comparisons), but not PGE2, PGF2alpha nor TXA2. Moreover, such an augumentation of PGI2 secretion by cyclic mechanical stretching was not observed in cultured human umbilical artery nor in cultured human coronary artery smooth muscle cells. These results suggest that cyclic mechanical stretching by labor, in concert with IL-1alpha stimulation, contributes to the increase in myometrial PGI2 secretion during delivery. |
| Databáze: | OpenAIRE |
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