Cardiovascular and respiratory activity of PK20, opioid and neurotensin hybrid peptide in anesthetized and awake rats
Autor: | Piotr Wojciechowski, Monika Jampolska, Patrycja Kleczkowska, Katarzyna Kaczyńska, Andrzej W. Lipkowski |
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Rok vydání: | 2017 |
Předmět: |
Male
0301 basic medicine Respiratory rate medicine.medical_treatment Intraperitoneal injection Blood Pressure 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Anesthesia Rats Wistar Respiratory system Tidal volume Pharmacology Dose-Response Relationship Drug Chemistry Respiration Vagotomy Rats 030104 developmental biology Blood pressure Breathing Oligopeptides Injections Intraperitoneal 030217 neurology & neurosurgery Neurotensin |
Zdroj: | European Journal of Pharmacology. 797:20-25 |
ISSN: | 0014-2999 |
Popis: | Aim Chimeric compound – PK20 despite its therapeutic activity on nociceptive and inflammatory processes may affect respiration and blood pressure. Our objective was to evaluate influence of the hybrid composed of endomorphin-2 and neurotensin fragments on ventilation, heart rate and blood pressure in anesthetized and awake rats. Methods The effects of PK20 (1 mg/kg) were studied either after its intravenous administration in anesthetized rats or intraperitoneal injection in awake state. Tidal volume and the timing components of the breathing pattern, arterial blood pressure, and heart rate were recorded. Results Intravenous administration of PK20 in the neurally intact rats evoked a dose-dependent apnoea followed by a transient insignificant increase in tidal volume and breathing rate. The blood pressure changes were biphasic: transient increase was replaced by prolonged hypotension. Midcervical vagotomy abrogated all post-PK20 respiratory effects. Hypotension was eliminated after blockade of neurotensin NTS 1 receptor, while respiratory changes were reduced by blockade of both: NTS 1 and μ opioid receptors. After PK20 intraperitoneal injection awake rats did not show any significant changes in ventilation and blood pressure. Conclusion This chimeric peptide should be used with care via intravenous administration in anesthetized animals since PK20 may evoke respiratory apnoea and hypotension. Nevertheless, applied intraperitoneally in the same dose in conscious rats induced no adverse effects. |
Databáze: | OpenAIRE |
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