Discriminating activated sludge flocs from biofilm microbial communities in a novel pilot-scale reciprocation MBR using high-throughput 16S rRNA gene sequencing
Autor: | Woonyoung Lee, Ryan De Sotto, Sungwoo Bae, Jaeho Ho |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Environmental Engineering Biofouling Candidatus Accumulibacter 010501 environmental sciences Management Monitoring Policy and Law Wastewater Membrane bioreactor 01 natural sciences Waste Disposal Fluid 03 medical and health sciences Bioreactors RNA Ribosomal 16S Bioreactor Waste Management and Disposal Effluent 0105 earth and related environmental sciences biology Bacteria Sewage Chemistry Chemical oxygen demand Biofilm Membranes Artificial General Medicine Pulp and paper industry biology.organism_classification 030104 developmental biology Biofilms Nitrospira |
Zdroj: | Journal of environmental management. 217 |
ISSN: | 1095-8630 |
Popis: | Membrane bioreactors (MBRs) are a well-established filtration technology that has become a popular solution for treating wastewater. One of the drawbacks of MBRs, however, is the formation of biofilm on the surface of membrane modules. The occurrence of biofilms leads to biofouling, which eventually compromises water quality and damages the membranes. To prevent this, it is vital to understand the mechanism of biofilm formation on membrane surfaces. In this pilot-scale study, a novel reciprocation membrane bioreactor was operated for a period of 8 months and fed with domestic wastewater from an aerobic tank of a local WWTP. Water quality parameters were monitored and the microbial composition of the attached biofilm and suspended aggregates was evaluated in this reciprocating MBR configuration. The abundance of nitrifiers and composition of microbial communities from biofilm and suspended solids samples were investigated using qPCR and high throughput 16S amplicon sequencing. Removal efficiencies of 29%, 16%, and 15% of chemical oxygen demand, total phosphorus and total nitrogen from the influent were observed after the MBR process with average effluent concentrations of 16 mg/L, 4.6 mg/L, and 5.8 mg/L respectively. This suggests that the energy-efficient MBR, apart from reducing the total energy consumption, was able to maintain effluent concentrations that are within regulatory standards for discharge. Molecular analysis showed the presence of amoA Bacteria and 16S Nitrospira genes with the occurrence of nitrification. Candidatus Accumulibacter, a genus with organisms that can accumulate phosphorus, was found to be present in both groups which explains why phosphorus removal was observed in the system. High-throughput 16S rRNA amplicon sequencing revealed the genus Saprospira to be the most abundant species from the total OTUs of both the membrane tank and biofilm samples. |
Databáze: | OpenAIRE |
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