Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation
Autor: | Dae Geun Han, Yongsung Hwang, Kook Yang Park, Kuk Hui Son, Chi Bum Ahn, Jin Woo Lee, Joo Hyun Kim, Ji-Hyun Lee |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Scaffold
Cell type Vascular smooth muscle Polymers and Plastics 0206 medical engineering 02 engineering and technology infiltration Article Extracellular matrix lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry medicine vascular scaffold electrospinning Chemistry Regeneration (biology) technology industry and agriculture General Chemistry 021001 nanoscience & nanotechnology medicine.disease musculoskeletal system 020601 biomedical engineering Electrospinning vascular smooth muscle Biophysics cardiovascular system 0210 nano-technology Infiltration (medical) Caprolactone optimization |
Zdroj: | Polymers, Vol 11, Iss 4, p 643 (2019) Polymers Volume 11 Issue 4 |
ISSN: | 2073-4360 |
Popis: | Due to the morphological resemblance between the electrospun nanofibers and extracellular matrix (ECM), electrospun fibers have been widely used to fabricate scaffolds for tissue regeneration. Relationships between scaffold morphologies and cells are cell type dependent. In this study, we sought to determine an optimum electrospun fiber diameter for human vascular smooth muscle cell (VSMC) regeneration in vascular scaffolds. Scaffolds were produced using poly(caprolactone) (PCL) electrospun fiber diameters of 0.5, 0.7, 1, 2, 2.5, 5, 7 or 10 &mu m, and VSMC survivals, proliferations, infiltrations, and phenotypes were recorded after culturing cells on these scaffolds for one, four, seven, or 10 days. VSMC phenotypes and macrophage infiltrations into scaffolds were evaluated by implanting scaffolds subcutaneously in a mouse for seven, 14, or 28 days. We found that human VSMC survival was not dependent on the electrospun fiber diameter. In summary, increasing fiber diameter reduced VSMC proliferation, increased VSMC infiltration and increased macrophage infiltration and activation. Our results indicate that electrospun PCL fiber diameters of 7 or 10 µ m are optimum in terms of VSMC infiltration and macrophage infiltration and activation, albeit at the expense of VSMC proliferation. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |