Sesquiterpene lactones from Geigeria aspera Harv. and their cytotoxicity
| Autor: | Dave C Liles, Y Zethu Mathe, Louis G J Ackerman, Gerda Fouche, E Annette Venter, Christo J. Botha |
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| Rok vydání: | 2019 |
| Předmět: |
Traditional medicine
Vermeersiekte 010405 organic chemistry Organic Chemistry Plant Science Sesquiterpene 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Geigeria aspera 010404 medicinal & biomolecular chemistry Plant Poisoning chemistry.chemical_compound chemistry Cytotoxicity |
| DOI: | 10.6084/m9.figshare.9989099.v1 |
| Popis: | Geigeria poisoning, referred to as ‘vermeersiekte’ is an important plant poisoning in southern Africa. Three sesquiterpene lactones, isogeigerin acetate (1) ivalin (2) and geigerin (3) were isolated and purified from Geigeria aspera Harv. (Asteraceae). Structures were deduced using 1 and 2D NMR spectroscopy and mass spectrometry, while the absolute configurations of compounds 1 and 3 were determined for the first time by X-ray crystal diffraction analyses. Cytotoxicity of isogeigerin acetate, ivalin and geigerin were compared by exposing a murine skeletal myoblast (C2C12) cell line to varying concentrations of the three sesquiterpene lactones isolated. Cell viability was assessed using the methyl-thiazolyl-tetrazolium (MTT) assay. The EC50s were 3.746, 0.0029 and 3.792 mM for isogeigerin acetate (1), ivalin (2) and geigerin (3), respectively. The results indicate that ivalin is much more toxic, approximately 1000 times, in vitro compared to isogeigerin acetate and geigerin. |
| Databáze: | OpenAIRE |
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