Isolation of Toxic Metabolites of Phomopsis leptostromiformis Responsible for Lupinosis
| Autor: | A B Beck, P. A. Cockrum, J. E. Peterson, L W Smith, J. A. Edgar, GW Lanigan, M. Clarke, M V Jago, C. C. J. Culvenor, AL Payne, R R White, D.S. Petterson, J L Frahn |
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| Rok vydání: | 1977 |
| Předmět: |
Electrophoresis
Chemical Phenomena Fungus In Vitro Techniques Endocrinology In vivo Diaporthe toxica Genetics Animals Organic chemistry Bioassay General Materials Science Countercurrent Distribution Molecular Biology Biological sciences Cells Cultured Plant Poisoning Phomopsis leptostromiformis Chromatography biology Chemistry General Medicine Mycotoxins Isolation (microbiology) biology.organism_classification Reproductive Medicine Cell culture Solvents Biological Assay Animal Science and Zoology Chromatography Thin Layer Mitosporic Fungi Developmental Biology Biotechnology |
| Zdroj: | Australian Journal of Biological Sciences. 30:269 |
| ISSN: | 0004-9417 |
| Popis: | Two metabolites of P. leptostromijormis (phomopsins A and B) have been isolated as a crystalline mixture from a culture of the fungus on lupin seed. The mixture has been shown to be capable of inducing lupinosis in sheep and in young rats. Key steps in the isolation were the transfer of the phomopsins from crude aqueous solution to tetrahydrofuran and chromatography on macroreticular polystyrene resin. The bioassays used in monitoring fractions were based on inhibition of cell cultures and the mitosis-arresting effect of the metabolites on liver cells in vivo. |
| Databáze: | OpenAIRE |
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