The control of UCP1 is dissociated from that of PGC-1α or of mitochondriogenesis as revealed by a study using β-less mouse brown adipocytes in culture
| Autor: | Jean-Paul Giacobino, Kriss Canola, Patrick Muzzin, Françoise Kuehne, Cedric S. Asensio, Lorenz Lehr, Maria Jimenez |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
medicine.medical_specialty
UCP1 Rodent Brown Adipocytes Blotting Western Biophysics PGC-1α Biology Mitochondriogenesis Polymerase Chain Reaction Biochemistry Ion Channels Mitochondrial Proteins Mice 03 medical and health sciences 0302 clinical medicine Adipose Tissue Brown Structural Biology Internal medicine biology.animal Receptors Adrenergic beta Brown adipose tissue Adipocytes Genetics medicine Animals RNA Messenger Molecular Biology Transcription factor Uncoupling Protein 1 DNA Primers 030304 developmental biology 0303 health sciences Messenger RNA Base Sequence Brown adipocytes Membrane Proteins Cell Biology TFAM Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Thermogenin β-less mice medicine.anatomical_structure Endocrinology Membrane protein Trans-Activators Carrier Proteins 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | FEBS Letters. (19):4661-4666 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2006.07.037 |
| Popis: | In rodent brown adipose tissue, the beta-adrenergic signaling is believed, by an action on PGC-1alpha, to control UCP1 expression and mitochondriogenesis. We addressed this hypothesis using beta(1)/beta(2)/beta(3)-adrenoceptor knockout (beta-less) brown adipocytes in primary culture. In these cells: (a) proliferation and differentiation into multilocular cells were normal; (b) UCP1 mRNA expression was dramatically decreased (by 93%), whereas PGC-1alpha and mtTFA mRNA expressions were not; (c) UCP1, PGC-1alpha and COX IV protein expressions were decreased by 97%, 62% and 22%, respectively. Altogether the data show a dissociation between the control of UCP1, which is mostly beta-adrenoceptor-dependent and that of PGC-1alpha and of mitochondriogenesis which are not. |
| Databáze: | OpenAIRE |
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